ArticleCell and tissue research1992
Differentiation of human skeletal muscle cells in culture: maturation as indicated by titin and desmin striation.
Article in Cell and tissue research, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 67 citations in OpenAlex.
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- IGF1 promotes human myotube differentiation toward a mature metabolic and contractile phenotype.American journal of physiology. Cell physiology · 2024Article
- Aligned skeletal muscle assembly on a biofunctionalized plant leaf scaffold.Acta biomaterialia · 2023Article
- Nanopattern surface improves cultured human myotube maturation.Skeletal muscle · 2021Article
- Tempora: Cell trajectory inference using time-series single-cell RNA sequencing data.PLoS computational biology · 2020Article
- Sarcoglycan Alpha Mitigates Neuromuscular Junction Decline in Aged Mice by Stabilizing LRP4.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Label-Free, High-Throughput Purification of Satellite Cells Using Microfluidic Inertial Separation.Tissue engineering. Part C, Methods · 2018Article
- Muscle Yap Is a Regulator of Neuromuscular Junction Formation and Regeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Cell Density and Joint microRNA-133a and microRNA-696 Inhibition Enhance Differentiation and Contractile Function of Engineered Human Skeletal Muscle Tissues.Tissue engineering. Part A · 2016Article
- Abnormalities of AMPK activation and glucose uptake in cultured skeletal muscle cells from individuals with chronic fatigue syndrome.PloS one · 2015Article
- Expression profiles of muscle disease-associated genes and their isoforms during differentiation of cultured human skeletal muscle cells.BMC musculoskeletal disorders · 2012Article
- Unidentified cells reside in fish skeletal muscle.Cytotechnology · 2008Article
- IP3 receptors and associated Ca2+ signals localize to satellite cells and to components of the neuromuscular junction in skeletal muscle.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Indications for a novel muscular dystrophy pathway. gamma-filamin, the muscle-specific filamin isoform, interacts with myotilin.The Journal of cell biology · 2000Article
- Thick filament assembly occurs after the formation of a cytoskeletal scaffold.Journal of muscle research and cell motility · 1999Article
- Expression of sarcomeric proteins and assembly of myofibrils in the putative myofibroblast cell line BHK-21/C13.Journal of muscle research and cell motility · 1998Article
- The identification of myogenic cells in skeletal muscle, with emphasis on the use of tritiated thymidine autoradiography and desmin antibodies.Journal of anatomy · 1998Review
- Dedifferentiation of atrial cardiomyocytes as a result of chronic atrial fibrillation.The American journal of pathology · 1997Article
- Human primary myoblast cell cultures from non-diabetic insulin resistant subjects retain defects in insulin action.The Journal of clinical investigation · 1996Article
- Titin expression as an early indication of heart and skeletal muscle differentiation in vitro. Developmental re-organisation in relation to cytoskeletal constituents.Journal of muscle research and cell motility · 1996Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This report describes a phenotyping study of differentiating human skeletal muscle cells in tissue culture. Satellite cells (adult myoblasts), isolated from biopsy material, showed a proliferative behaviour in high-nutrition medium, but fused to form myotubes when grown in low-nutrition medium. The expression and structural organization of the intermediate filament proteins desmin and vimentin as well as the sarcomeric constituents alpha-actin, alpha-actinin, nebulin, myosin and especially titin during myofibrillogenesis in vitro, were studied by means of indirect immunofluorescence assays. The proliferating myoblasts contained both desmin and vimentin, alpha-actinin and the filamentous form of actin. Shortly after the change of medium, expression of titin, sarcomeric myosin and skeletal muscle alpha-actin was found in mononuclear cells in a diffuse, filamentous (titin, myosin, alpha-actin) or punctate (titin, myosin) pattern. Four to 10 days after the medium change, mature myotubes showed desmin, titin, alpha-actinin, nebulin, sarcomeric myosin and actin cross-striations, while vimentin was no longer detected. We conclude that human skeletal muscle cell cultures are an appropriate model system to study the molecular basis of myofibrillogenesis. Especially the presence of desmin in a striated fashion points to a high degree of maturation of the muscle cell cultures.
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