ArticleMolecular biology of the cell1992
Myosin II phosphorylation and the dynamics of stress fibers in serum-deprived and stimulated fibroblasts.
Article in Molecular biology of the cell, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- Lamin A/C deficiency enables increased myosin-II bipolar filament ensembles that promote divergent actomyosin network anomalies through self-organization.Molecular biology of the cell · 2020Article
- Actomyosin contractility confers mechanoprotection against TNFα-induced disruption of the intervertebral disc.Science advances · 2020Article
- Emerin organizes actin flow for nuclear movement and centrosome orientation in migrating fibroblasts.Molecular biology of the cell · 2013Article
- Specific isoforms of drosophila shroom define spatial requirements for the induction of apical constriction.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Article
- Proteomic profiling of glucocorticoid-exposed myogenic cells: Time series assessment of protein translocation and transcription of inactive mRNAs.Proteome science · 2009Article
- A molecular switch that controls cell spreading and retraction.The Journal of cell biology · 2007Article
- Simultaneous stretching and contraction of stress fibers in vivo.Molecular biology of the cell · 2004Article
- Rho-kinase-mediated Ca2+-independent contraction in rat embryo fibroblasts.American journal of physiology. Cell physiology · 2004Article
- Activation of myosin phosphatase targeting subunit by mitosis-specific phosphorylation.The Journal of cell biology · 1999Article
- Ca2+-independent myosin II phosphorylation and contraction in chicken embryo fibroblasts.The Journal of physiology · 1999Article
- Specific localization of serine 19 phosphorylated myosin II during cell locomotion and mitosis of cultured cells.The Journal of cell biology · 1998Article
- Regulation of cell motility by mitogen-activated protein kinase.The Journal of cell biology · 1997Article
- Expression of a novel myosin light chain kinase in embryonic tissues and cultured cells.The Journal of biological chemistry · 1995Article
- A fluorescent protein biosensor of myosin II regulatory light chain phosphorylation reports a gradient of phosphorylated myosin II in migrating cells.Molecular biology of the cell · 1995Article
- Contraction due to microtubule disruption is associated with increased phosphorylation of myosin regulatory light chain.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Inhibition of lysophosphatidate- and thrombin-induced neurite retraction and neuronal cell rounding by ADP ribosylation of the small GTP-binding protein Rho.The Journal of cell biology · 1994Article
- Fluorescent actin analogs with a high affinity for profilin in vitro exhibit an enhanced gradient of assembly in living cells.The Journal of cell biology · 1994Article
- In vitro models of tail contraction and cytoplasmic streaming in amoeboid cells.The Journal of cell biology · 1993Article
- Gradients in the concentration and assembly of myosin II in living fibroblasts during locomotion and fiber transport.Molecular biology of the cell · 1993Article
- Fluorescence anisotropy imaging microscopy maps calmodulin binding during cellular contraction and locomotion.The Journal of cell biology · 1993Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The actin-based cytomatrix generates stress fibers containing a host of proteins including actin and myosin II and whose dynamics are easily observable in living cells. We developed a dual-radioisotope-based assay of myosin II phosphorylation and applied it to serum-deprived fibroblasts treated with agents that modified the dynamic distribution of stress fibers and/or altered the phosphorylation state of myosin II. Serum-stimulation induced an immediate and sustained increase in the level of myosin II heavy chain (MHC) and 20-kDa light chain (LC20) phosphorylation over the same time course that it caused stress fiber contraction. Cytochalasin D, shown to cause stress fiber fragmentation and contraction, had little effect on myosin II phosphorylation. Okadaic acid, a protein phosphatase inhibitor, induced a delayed but massive cell shortening preceded by a large increase in MHC and LC20 phosphorylation. Staurosporine, a kinase inhibitor known to effect dissolution but not contraction of stress fibers, immediately caused an increase in MHC and LC20 phosphorylation followed within minutes by the dephosphorylation of LC20 to a level below that of untreated cells. We therefore propose that the contractility of the actin-based cytomatrix is regulated by both modulating the activity of molecular motors such as myosin II and by altering the gel structure in such a manner as to either resist or yield to the tension applied by the motors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.