ArticleMolecular biology of the cell1992
Interaction of dynamin with microtubules: its structure and GTPase activity investigated by using highly purified dynamin.
Article in Molecular biology of the cell, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 113 citations in OpenAlex.
- Dynamins maintain nuclear envelope homeostasis and genome stability.Nature communications · 2026Article
- Membrane remodeling properties of the Parkinson's disease protein LRRK2.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- The Lipid-Binding Defective Dynamin 2 Mutant in Charcot-Marie-Tooth Disease Impairs Proper Actin Bundling and Actin Organization in Glomerular Podocytes.Frontiers in cell and developmental biology · 2022Article
- Dynamin Inhibitors Prevent the Establishment of the Cytomegalovirus Assembly Compartment in the Early Phase of Infection.Life (Basel, Switzerland) · 2021Article
- Disparate Entry of Adenoviruses Dictates Differential Innate Immune Responses on the Ocular Surface.Microorganisms · 2019Review
- Multiomics analyses of vesicular transport pathway-specific transcripts and proteins in ovine amnion: responses to altered intramembranous transport.Physiological genomics · 2019Article
- Structural Insights into the Mechanism of Dynamin Superfamily Proteins.Trends in cell biology · 2019Review
- Impact of dynamin 2 on adenovirus nuclear entry.Virology · 2019Article
- Dynamin impacts homology-directed repair and breast cancer response to chemotherapy.The Journal of clinical investigation · 2018Article
- Actin dynamics at focal adhesions: a common endpoint and putative therapeutic target for proteinuric kidney diseases.Kidney international · 2018Review
- Dynamics of the sealing zone in cultured osteoclasts.Cytoskeleton (Hoboken, N.J.) · 2017Article
- Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis.The Plant cell · 2015Article
- SH3 Domains Differentially Stimulate Distinct Dynamin I Assembly Modes and G Domain Activity.PloS one · 2015Article
- Regulation of dynamin oligomerization in cells: the role of dynamin-actin interactions and its GTPase activity.Traffic (Copenhagen, Denmark) · 2014Article
- Building a better dynasore: the dyngo compounds potently inhibit dynamin and endocytosis.Traffic (Copenhagen, Denmark) · 2013Article
- Dynamin rings: not just for fission.Traffic (Copenhagen, Denmark) · 2013Review
- Dynamin-2 function and dysfunction along the secretory pathway.Frontiers in endocrinology · 2013Review
- Disruption of microtubule network rescues aberrant actin comets in dynamin2-depleted cells.PloS one · 2011Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We purified a large amount of dynamin with high enzymatical activity from rat brain tissue by a new procedure. Dynamin 0.48 mg was obtained from 20 g of rat brain. The purity of dynamin was almost 98%. Dynamin plays a role of GTPase rather than ATPase. In the absence of microtubules, Michaelis constant (Km) and maximum velocity (Vmax) for dynamin GTPase were 370 microM and 0.25 min-1, respectively, and in their presence, both were significantly accelerated up to 25 microM and 5.5 min-1. On the other hand, the ATPase activity was very low in the absence of microtubules, and even in their presence, Km and Vmax for dynamin ATPase were 0.2 mM and 0.91 min-1. Despite slow GTPase turnover rate in the absence of microtubules, binding of GTP and its nonhydrolizing analogues was very fast, indicating that GTP binding step is not rate limiting. Dynamin did not cause a one-directional consistent microtubule sliding movement just like kinesin or dynein in the presence of 2 mM ATP or 2 mM GTP. We observed the molecular structure of dynamin with low-angle rotary shadowing technique and revealed that the dynamin molecule is globular in shape. Gel filtration assay revealed that these globules were the oligomers of 100-kDa dynamin polypeptide. Dynamin bound to microtubules with a 1:1 approximately 1.2 molar ratio in the absence of GTP. Quick-freeze deep-etch electron microscopy of the dynamin-microtubule complex showed that dynamin decorates the surface of microtubules helically, like a screw bolt, very orderly and tightly with 11.4 +/- 0.9 (SD)nm period. Contrary to the previous report, microtubules make bundles by the attachment of the dynamin helixes around each adjacent microtubule, and no cross-bridge formation was observed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.