ArticleProceedings of the National Academy of Sciences of the United States of America1992
Myristoylation of the G alpha i2 polypeptide, a G protein alpha subunit, is required for its signaling and transformation functions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 45 citations in OpenAlex.
- Every Detail Matters. That Is, How the Interaction between Gα Proteins and Membrane Affects Their Function.Membranes · 2021Review
- Article
- Effects of Post-translational Modifications on Membrane Localization and Signaling of Prostanoid GPCR-G Protein Complexes and the Role of Hypoxia.The Journal of membrane biology · 2019Review
- GPS-Lipid: a robust tool for the prediction of multiple lipid modification sites.Scientific reports · 2016Article
- Myristoylation exerts direct and allosteric effects on Gα conformation and dynamics in solution.Biochemistry · 2012Article
- Molecular mechanisms of go signaling.Neuro-Signals · 2009Review
- Galpha subunit Gpa2 recruits kelch repeat subunits that inhibit receptor-G protein coupling during cAMP-induced dimorphic transitions in Saccharomyces cerevisiae.Molecular biology of the cell · 2005Article
- Regulation of G proteins by covalent modification.Oncogene · 2001Review
- Mapping the interface between calmodulin and MARCKS-related protein by fluorescence spectroscopy.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Effect of thyroid deficiency on Go alpha-subunit isoforms in developing rat cerebral cortex.Neurochemical research · 1995Article
- Regulation of G-protein alpha i2 subunit expression by oxidized low-density lipoprotein.The Journal of clinical investigation · 1995Article
- N-terminal fatty acylation of the alpha-subunit of the G-protein Gi1: only the myristoylated protein is a substrate for palmitoylation.The Biochemical journal · 1994Article
- The palmitoylation status of the G-protein G(o)1 alpha regulates its activity of interaction with the plasma membrane.The Biochemical journal · 1994Article
- Pheromone action regulates G-protein alpha-subunit myristoylation in the yeast Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Characterization of the myristylated polypeptide encoded by the UL1 gene that is conserved in the genome of defective interfering particles of equine herpesvirus 1.Journal of virology · 1993Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
GTPase-inhibiting mutations of the alpha subunit (alpha i2) of the G protein, Gi2, result in constitutive activation of alpha i2 signal transduction functions. GTPase-inhibited alpha i2 mutant polypeptides, referred to as gip2 oncoproteins, have glutamine-205 mutated to leucine (alpha i2Q205L). Expression of the alpha i2Q205L polypeptide inhibits adenylyl cyclase stimulation, constitutively activates p42 mitogen-activated protein kinase, and transforms Rat 1a fibroblasts. The alpha i2 polypeptides are N-terminal-myristoylated, but the function of myristoylation is unclear in alpha i2 signal transduction. We have tested the requirement for myristoylation on the ability of the alpha i2Q205L mutant polypeptide to constitutively regulate signal pathways and cell transformation. When expressed in Rat 1a cells, the nonmyristoylated alpha i2Q205L polypeptide is membrane associated but is unable to regulate adenylyl cyclase or p42 mitogen-activated protein kinase and does not induce cellular transformation. We conclude that myristoylation is absolutely necessary for alpha i2Q205L signal transduction and regulation of effector enzymes in the cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.