ArticleProceedings of the National Academy of Sciences of the United States of America1992
Efficient mass transformation of Tetrahymena thermophila by electroporation of conjugants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 123 citations in OpenAlex.
- Development of a Tetrahymena thermophila-Based Vaccine Expressing Miamiensis avidus Ciliary Proteins to Combat Scuticociliatosis.Journal of fish diseases · 2025Article
- Molecular Cloning, Expression and Enzymatic Characterization of Tetrahymena thermophila Glutathione-S-Transferase Mu 34.The protein journal · 2024Article
- Application of RNA interference and protein localization to investigate housekeeping and developmentally regulated genes in the emerging model protozoan Paramecium caudatum.Communications biology · 2024Article
- PIWI-Directed DNA Elimination for Tetrahymena Genetics.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Efficient expression of codon-adapted affinity tagged super folder green fluorescent protein for synchronous protein localization and affinity purification studies in Tetrahymena thermophila.BMC biotechnology · 2015Article
- Functional GFP-metallothionein fusion protein from Tetrahymena thermophila: a potential whole-cell biosensor for monitoring heavy metal pollution and a cell model to study metallothionein overproduction effects.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2014Article
- Distinct functional roles of β-tubulin isotypes in microtubule arrays of Tetrahymena thermophila, a model single-celled organism.PloS one · 2012Article
- Comprehensive analysis reveals dynamic and evolutionary plasticity of Rab GTPases and membrane traffic in Tetrahymena thermophila.PLoS genetics · 2010Article
- Histone H2B ubiquitylation is not required for histone H3 methylation at lysine 4 in tetrahymena.The Journal of biological chemistry · 2009Article
- Abnormal micronuclear telomeres lead to an unusual cell cycle checkpoint and defects in Tetrahymena oral morphogenesis.Eukaryotic cell · 2008Article
- A class II histone deacetylase acts on newly synthesized histones in Tetrahymena.Eukaryotic cell · 2008Article
- Combination of two regulatory elements in the Tetrahymena thermophila HSP70-1 gene controls heat shock activation.Eukaryotic cell · 2008Article
- The bifunctional dihydrofolate reductase thymidylate synthase of Tetrahymena thermophila provides a tool for molecular and biotechnology applications.BMC biotechnology · 2006Article
- Biochemical and molecular characterisation of Tetrahymena thermophila extracellular cysteine proteases.BMC microbiology · 2006Article
- Responses of the ciliates Tetrahymena and Paramecium to external ATP and GTP.Purinergic signalling · 2005Article
- Class I histone deacetylase Thd1p affects nuclear integrity in Tetrahymena thermophila.Eukaryotic cell · 2005Article
- Role of micronucleus-limited DNA in programmed deletion of mse2.9 during macronuclear development of Tetrahymena thermophila.Eukaryotic cell · 2004Article
- Diverse sequences within Tlr elements target programmed DNA elimination in Tetrahymena thermophila.Eukaryotic cell · 2003Article
- Loss of cap structure causes mitotic defect in Tetrahymena thermophila telomerase mutants.Chromosoma · 2003Article
- New class of cargo protein in Tetrahymena thermophila dense core secretory granules.Eukaryotic cell · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Conjugating cells of the ciliate Tetrahymena thermophila were electroporated in the presence of plasmid DNA containing a paromomycin-resistant ribosomal RNA gene (rDNA). Cells were selected with paromomycin following 12-24 hr of growth on nonselective medium. Resistant cells appeared after 2-3 days. Processing vectors containing the micronuclear rDNA and somatic vectors containing the macronuclear gene transformed the cells, with the former yielding frequencies up to 900 transformants per microgram of plasmid DNA. A ribosomal protein gene (rpL29) conferring cycloheximide resistance also transformed conjugating cells. The transformation efficiency of the plasmid containing only the rpL29 gene was increased by insertion of an rDNA replication origin and by cotransformation and preselection with an rDNA vector. These results indicate that electroporation can be used for the production of large numbers of transformed Tetrahymena.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.