ArticlePflugers Archiv : European journal of physiology1992
Ion conductances of isolated cortical collecting duct cells.
Article in Pflugers Archiv : European journal of physiology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Review
- Harvest and primary culture of the murine aldosterone-sensitive distal nephron.American journal of physiology. Renal physiology · 2015Article
- Protective role of NHE-3 inhibition in rat renal transplantation undergoing acute rejection.Pflugers Archiv : European journal of physiology · 2008Article
- K depletion increases protein tyrosine kinase-mediated phosphorylation of ROMK.American journal of physiology. Renal physiology · 2002Article
- K+ channels in the basolateral membrane of rat cortical collecting duct are regulated by a cGMP-dependent protein kinase.Pflugers Archiv : European journal of physiology · 1995Article
- Effect of NH4+/NH3 on cytosolic pH and the K+ channels of freshly isolated cells from the thick ascending limb of Henle's loop.Pflugers Archiv : European journal of physiology · 1995Article
- pH dependence of K+ conductances of rat cortical collecting duct principal cells.Pflugers Archiv : European journal of physiology · 1994Article
- K+ channels in the basolateral membrane of rat cortical collecting duct.Pflugers Archiv : European journal of physiology · 1993Article
- Regulation and possible physiological role of the Ca(2+)-dependent K+ channel of cortical collecting ducts of the rat.Pflugers Archiv : European journal of physiology · 1993Article
- Cation specificity and pharmacological properties of the Ca(2+)-dependent K+ channel of rat cortical collecting ducts.Pflugers Archiv : European journal of physiology · 1993Article
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3 authors.
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Abstract
The study of ion conductances in the intact cortical collecting duct (CCD) with the patch-clamp method is rather difficult. An optimized method to isolate CCD cells from rat kidneys using an in vivo followed by an in vitro enzyme digestion is described. Individual CCD segments were collected after this digestion and incubated in EGTA-buffered medium. This procedure resulted in single cells or cell clusters. These freshly isolated CCD cells were studied with different modifications of the patch-clamp method. Membrane voltages measured in the cell-attached-nystatin configuration were -74 +/- 1 mV (n = 13) and -68 +/- 3 mV (n = 22) in cells isolated from normal and mineralocorticoid-treated rats respectively. These values and those measured with the nystatin-perforated slow-whole-cell configuration (-79 +/- 1 mV, n = 23) are comparable to those measured in principal cells of isolated CCD segments. The cells hyperpolarized after the addition of amiloride and depolarized with the addition of adiuretin to the bath. The amiloride effect was enhanced when cells were isolated from deoxycorticosterone-acetate-treated rats. The cells were strongly depolarized upon elevation of the extracellular K(+)-concentration and did not demonstrate a measurable Cl- conductance. A large-conductance K+ channel (174 pS, n = 5, cell-attached, 145 mmol/l K+ in the pipette; 140 pS, n = 12, cell-free, 3.6 mmol/l K+ in the bath) was seen. It had a very low activity on the cell, but a high open probability when excised into a solution with 1 mmol/l Ca2+ on the cytosolic side.(ABSTRACT TRUNCATED AT 250 WORDS)
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