ArticleMolecular and cellular biology1992
Elements responsible for hormonal control and tissue specificity of L-type pyruvate kinase gene expression in transgenic mice.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 48 citations in OpenAlex.
- Hepatic lipid metabolism is affected by a daily 3-meal pattern with varying dietary crude protein with a pig model.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2020Article
- Salt-inducible kinase regulates hepatic lipogenesis by controlling SREBP-1c phosphorylation.The Journal of biological chemistry · 2009Article
- New perspectives in the regulation of hepatic glycolytic and lipogenic genes by insulin and glucose: a role for the transcription factor sterol regulatory element binding protein-1c.The Biochemical journal · 2002Review
- Hepatic nuclear factor 1-alpha directs nucleosomal hyperacetylation to its tissue-specific transcriptional targets.Molecular and cellular biology · 2001Article
- Nutrient and hormonal regulation of pyruvate kinase gene expression.The Biochemical journal · 1999Review
- The maturity-onset diabetes of the young (MODY1) transcription factor HNF4alpha regulates expression of genes required for glucose transport and metabolism.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Members of the nuclear factor 1 family and hepatocyte nuclear factor 4 bind to overlapping sequences of the L-II element on the rat pyruvate kinase L gene promoter and regulate its expression.The Biochemical journal · 1997Article
- Estradiol-dependent uterine leiomyomas in transgenic mice.The Journal of clinical investigation · 1996Article
- Prevention of diabetic alterations in transgenic mice overexpressing Myc in the liver.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- An ATP-dependent As(III)-glutathione transport system in membrane vesicles of Leishmania tarentolae.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Tissue specificity of L-pyruvate kinase transgenes results from the combinatorial effect of proximal promoter and distal activator regions.Gene expression · 1996Article
- Tissue specificity of a glucocorticoid-dependent enhancer in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- The orphan receptors COUP-TF and HNF-4 serve as accessory factors required for induction of phosphoenolpyruvate carboxykinase gene transcription by glucocorticoids.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Transcriptional control of genes that regulate glycolysis and gluconeogenesis in adult liver.The Biochemical journal · 1994Review
- Functional characterization of the L-type pyruvate kinase gene glucose response complex.Molecular and cellular biology · 1993Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L-type pyruvate kinase (L-PK) is a key enzyme of the glycolytic pathway specifically expressed in the liver and, to a lesser degree, in the small intestine and kidney. One important characteristic of L-PK gene expression is its strong activation by glucose and insulin and its complete inhibition by fasting or glucagon treatment. Having previously established that the entire rat L-PK gene plus 3.2 kbp of 5'-flanking region functions in mice in a tissue-specific and hormonally regulated manner, various deletions of these 3.2 kbp of 5'-flanking regions were tested in transgenic animals to map the cis-acting elements involved in transcriptional gene regulation. Our experiments indicate that the proximal region between -183 and +11 confers tissue specificity and contains all the information necessary for dietary and hormonal control of L-PK gene expression in vivo. We found, however, that the transcriptional activity generated by this proximal promoter fragment can be modulated by distal sequences in a tissue-specific manner. (i) Sequences between bp -183 and -392 seem to play a dual role in the liver and small intestine; they induce L-PK expression in the liver but repress it in the small intestine. (ii) Sequences from bp -392 up to -1170 do not seem to have any additional effect on promoter activity. (iii) Between bp -1170 and -2080, we found a putative extinguisher whose transcriptional inhibitory effect is much more marked in the small intestine than in the liver. (iv) Finally, between bp -2080 and -3200, we identified an activating sequence required for full expression of the gene in the liver.
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