ArticleMolecular and cellular biology1992
Regulation of collagen I gene expression by ras.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 63 citations in OpenAlex.
- Ha-Ras stabilization mediates pro-fibrotic signals in dermal fibroblasts.Fibrogenesis & tissue repair · 2011Article
- Involvement of 90-kuD ribosomal S6 kinase in collagen type I expression in rat hepatic fibrosis.World journal of gastroenterology · 2009Article
- Overexpressed vs mutated Kras in murine fibroblasts: a molecular phenotyping study.British journal of cancer · 2009Article
- Enhanced prolidase activity and decreased collagen content in breast cancer tissue.International journal of experimental pathology · 2006Article
- Article
- Transcriptional basis of KRAS oncogene-mediated cellular transformation in ovarian epithelial cells.Oncogene · 2004Article
- Collagen metabolism disturbances are accompanied by an increase in prolidase activity in lung carcinoma planoepitheliale.International journal of experimental pathology · 2000Article
- The 5' stem-loop regulates expression of collagen alpha1(I) mRNA in mouse fibroblasts cultured in a three-dimensional matrix.Nucleic acids research · 2000Article
- Integrin signaling in fibrosis and scleroderma.Current rheumatology reports · 1999Review
- The Nf1 tumor suppressor regulates mouse skin wound healing, fibroblast proliferation, and collagen deposited by fibroblasts.The Journal of investigative dermatology · 1999Article
- Transcriptional and posttranscriptional silencing of rodent alpha1(I) collagen by a homologous transcriptionally self-silenced transgene.Molecular and cellular biology · 1999Article
- Regulation of type I collagen mRNA in lung fibroblasts by cystine availability.The Biochemical journal · 1998Article
- Tissue specific and vitamin D responsive gene expression in bone.Molecular biology reports · 1998Review
- Posttranscriptional regulation of collagen alpha1(I) mRNA in hepatic stellate cells.Molecular and cellular biology · 1997Article
- Okadaic acid-induced transcriptional downregulation of type I collagen gene expression is mediated by protein phosphatase 2A.Molecular and cellular biochemistry · 1996Article
- Functional analysis of the promoter and first intron of the human lysyl oxidase gene.Molecular biology reports · 1996Article
- Oncogenic Ras activates c-Jun via a separate pathway from the activation of extracellular signal-regulated kinases.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- A novel mechanism of Ha-ras oncogene action: regulation of fibronectin mRNA levels by a nuclear posttranscriptional event.Molecular and cellular biology · 1994Article
- Transforming growth factor beta and the cell surface in tumor progression.Cancer metastasis reviews · 1993Review
- RNA protein interactions governing expression of the most abundant protein in human body, type I collagen.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Although transformation of rodent fibroblasts can lead to dramatic changes in expression of extracellular matrix genes, the molecular basis and physiological significance of these changes remain poorly understood. In this study, we have investigated the mechanism(s) by which ras affects expression of the genes encoding type I collagen. Levels of both alpha 1(I) and alpha 2(I) collagen mRNAs were markedly reduced in Rat 1 fibroblasts overexpressing either the N-rasLys-61 or the Ha-rasVal-12 oncogene. In fibroblasts conditionally transformed with N-rasLys-61, alpha 1(I) transcript levels began to decline within 8 h of ras induction and reached 1 to 5% of control levels after 96 h. In contrast, overexpression of normal ras p21 had no effect on alpha 1(I) or alpha 2(I) mRNA levels. Nuclear run-on experiments demonstrated that the transcription rates of both the alpha 1(I) and alpha 2(I) genes were significantly reduced in ras-transformed cells compared with those in parental cells. In addition, the alpha 1(I) transcript was less stable in transformed cells. Chimeric plasmids containing up to 3.6 kb of alpha 1(I) 5'-flanking DNA and up to 2.3 kb of the 3'-flanking region were expressed at equivalent levels in both normal and ras-transformed fibroblasts. However, a cosmid clone containing the entire mouse alpha 1(I) gene, including 3.7 kb of 5'- and 4 kb of 3'-flanking DNA, was expressed at reduced levels in fibroblasts overexpressing oncogenic ras. We conclude that oncogenic ras regulates the type I collagen genes at both transcriptional and posttranscriptional levels and that this effect, at least for the alpha 1(I) gene, may be mediated by sequences located either within the body of the gene itself or in the distal 3'-flanking region.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.