ArticleThe Journal of cell biology1992
Astral microtubules are not required for anaphase B in Saccharomyces cerevisiae.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
100 citing papers in PubMed, 171 citations in OpenAlex.
- Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation.PLoS biology · 2025Article
- Expansion microscopy reveals characteristic ultrastructural features of pathogenic budding yeast species.Journal of cell science · 2024Article
- Epistasis, aneuploidy, and functional mutations underlie evolution of resistance to induced microtubule depolymerization.The EMBO journal · 2021Article
- Spatio-temporal regulation of nuclear division by Aurora B kinase Ipl1 in Cryptococcus neoformans.PLoS genetics · 2019Article
- Influence of the bud neck on nuclear envelope fission in Saccharomyces cerevisiae.Experimental cell research · 2017Article
- Anaphase B.Biology · 2016Review
- Article
- Cell polarization and cytokinesis in budding yeast.Genetics · 2012Review
- Mitotic spindle form and function.Genetics · 2012Article
- A novel patch assembly domain in Num1 mediates dynein anchoring at the cortex during spindle positioning.The Journal of cell biology · 2012Article
- Pronounced and extensive microtubule defects in a Saccharomyces cerevisiae DIS3 mutant.Yeast (Chichester, England) · 2011Article
- Studying G2 DNA damage checkpoints using the fission yeast Schizosaccharomyces pombe.Methods in molecular biology (Clifton, N.J.) · 2011Article
- The perpetual movements of anaphase.Cellular and molecular life sciences : CMLS · 2010Review
- An overview of Cdk1-controlled targets and processes.Cell division · 2010Article
- The spindle positioning protein Kar9p interacts with the sumoylation machinery in Saccharomyces cerevisiae.Genetics · 2008Article
- Stable preanaphase spindle positioning requires Bud6p and an apparent interaction between the spindle pole bodies and the neck.Eukaryotic cell · 2007Article
- The cyclin-dependent kinase Cdc28p regulates multiple aspects of Kar9p function in yeast.Molecular biology of the cell · 2007Article
- Synthesis and function of membrane phosphoinositides in budding yeast, Saccharomyces cerevisiae.Biochimica et biophysica acta · 2007Review
- Katanin controls mitotic and meiotic spindle length.The Journal of cell biology · 2006Article
- The spindle position checkpoint in budding yeast: the motherly care of MEN.Cell division · 2006Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
tub2-401 is a cold-sensitive allele of TUB2, the sole gene encoding beta-tubulin in the yeast, Saccharomyces cerevisiae. At 18 degrees C, tub2-401 cells are able to assemble spindle microtubules but lack astral microtubules. Under these conditions, movement of the spindle to the bud neck is blocked. However, spindle elongation and chromosome separation are unimpeded and occur entirely within the mother cell. Subsequent cytokinesis produces one cell with two nuclei and one cell without a nucleus. The anucleate daughter can not bud. The binucleate daughter proceeds through another cell cycle to produce a cell with four nuclei and another anucleate cell. With additional time in the cold, the number of nuclei in the nucleated cells continues to increase and the percentage of anucleate cells in the population rises. The results indicate that astral microtubules are needed to position the spindle in the bud neck but are not required for spindle elongation at anaphase B. In addition, cell cycle progression does not depend on the location or orientation of the spindle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.