ArticleThe Journal of cell biology1992
Comparison of actin and cell surface dynamics in motile fibroblasts.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed, 146 citations in OpenAlex.
- Haplotype-resolved genome assemblies of BJ and IMR-90 human fibroblast cell lines reveal extensive structural variation and enable reanalysis of historical sequencing data.Nucleic acids research · 2026Article
- Diploid genome assembly of human fibroblast cell lines enables clone specific variant calling, improved read mapping and accurate phasing.bioRxiv : the preprint server for biology · 2025Article
- Mechanisms of actin disassembly and turnover.The Journal of cell biology · 2023Review
- Extraction of accurate cytoskeletal actin velocity distributions from noisy measurements.Nature communications · 2022Article
- Article
- Coupling of βNature cell biology · 2019Article
- Prediction of traction forces of motile cells.Interface focus · 2016Article
- Extending the molecular clutch beyond actin-based cell motility.New journal of physics · 2014Article
- New single-molecule speckle microscopy reveals modification of the retrograde actin flow by focal adhesions at nanometer scales.Molecular biology of the cell · 2014Article
- Visualization of cytoskeletal dynamics in podocytes using adenoviral vectors.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Competition and collaboration between different actin assembly pathways allows for homeostatic control of the actin cytoskeleton.Bioarchitecture · 2014Article
- Actin dynamics affect mitochondrial quality control and aging in budding yeast.Current biology : CB · 2013Article
- Fibroblasts and the ground they walk on.Physiology (Bethesda, Md.) · 2013Review
- Polarization of actin cytoskeleton is reduced in dendritic protrusions during early spine development in hippocampal neuron.Molecular biology of the cell · 2012Article
- Diffusion, capture and recycling of SCAR/WAVE and Arp2/3 complexes observed in cells by single-molecule imaging.Journal of cell science · 2012Article
- Analysis of actin FLAP dynamics in the leading lamella.PloS one · 2010Article
- Force transmission in migrating cells.The Journal of cell biology · 2010Article
- Mechanical integration of actin and adhesion dynamics in cell migration.Annual review of cell and developmental biology · 2010Review
- Actin dynamics: from nanoscale to microscale.Annual review of biophysics · 2010Review
- Cellular symmetry breaking during Caenorhabditis elegans development.Cold Spring Harbor perspectives in biology · 2009Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the dynamic behavior of actin in fibroblast lamellipodia using photoactivation of fluorescence. Activated regions of caged resorufin (CR)-labeled actin in lamellipodia of IMR 90 and MC7 3T3 fibroblasts were observed to move centripetally over time. Thus in these cells, actin filaments move centripetally relative to the substrate. Rates were characteristic for each cell type; 0.66 +/- 0.27 microns/min in IMR 90 and 0.36 +/- 0.16 microns/min in MC7 3T3 cells. In neither case was there any correlation between the rate of actin movement and the rate of lamellipodial protrusion. The half-life of the activated CR-actin filaments was approximately 1 min in IMR 90 lamellipodia, and approximately 3 min in MC7 3T3 lamellipodia. Thus continuous filament turnover accompanies centripetal movement. In both cell types, the length of time required for a section of the actin meshwork to traverse the lamellipodium was several times longer than the filament half-life. The dynamic behavior of the dorsal surface of the cell was also observed by tracking lectin-coated beads on the surface and phase-dense features within lamellipodia of MC7 3T3 cells. The movement of these dorsal features occurred at rates approximately three times faster than the rate of movement of the underlying bulk actin cytoskeleton, even when measured in the same individual cells. Thus the transport of these dorsal features must occur by some mechanism other than simple attachment to the moving bulk actin cytoskeleton.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.