ArticleInfection and immunity1992
Electrophoretic karyotypes of clinical isolates of Coccidioides immitis.
Article in Infection and immunity, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Coccidioides Species: A Review of Basic Research: 2022.Journal of fungi (Basel, Switzerland) · 2022Review
- A chromosomal-level reference genome of the widely utilized Coccidioides posadasii laboratory strain "Silveira".G3 (Bethesda, Md.) · 2022Article
- Coccidioides immitis and posadasii; A review of their biology, genomics, pathogenesis, and host immunity.Virulence · 2018Review
- Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives.Genome research · 2009Article
- Amplification of coccidioidal DNA in clinical specimens by PCR.Journal of clinical microbiology · 2004Article
- A recombinant beta-1,3-glucanosyltransferase homolog of Coccidioides posadasii protects mice against coccidioidomycosis.Infection and immunity · 2003Article
- A parasitic phase-specific adhesin of Coccidioides immitis contributes to the virulence of this respiratory Fungal pathogen.Infection and immunity · 2002Article
- Cloning and expression of the gene which encodes a tube precipitin antigen and wall-associated beta-glucosidase of Coccidioides immitis.Infection and immunity · 2001Article
- Disruption of the gene which encodes a serodiagnostic antigen and chitinase of the human fungal pathogen Coccidioides immitis.Infection and immunity · 2000Article
- The ins and outs of DNA fingerprinting the infectious fungi.Clinical microbiology reviews · 2000Review
- Developments in fungal taxonomy.Clinical microbiology reviews · 1999Review
- The evolutionary biology and population genetics underlying fungal strain typing.Clinical microbiology reviews · 1999Review
- Electrophoretic karyotypes and genome sizing of the pathogenic fungus Paracoccidioides brasiliensis.Journal of clinical microbiology · 1998Article
- Molecular and biochemical characterization of a Coccidioides immitis-specific antigen.Infection and immunity · 1995Article
- Characterization of Coccidioides immitis isolates by restriction fragment length polymorphisms.Journal of clinical microbiology · 1994Article
- Karyotyping of Cryptococcus neoformans as an epidemiological tool.Journal of clinical microbiology · 1993Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
Abstract
Chromosomes of the fungal respiratory pathogen, Coccidioides immitis, were separated by contour-clamped homogeneous electric field gel electrophoresis. Twelve isolates were examined, the majority of which showed four chromosomes with a range of molecular size from 11.5 to 3.2 Mb. Three isolates (C634, C735, and L) revealed three chromosomal bands under the conditions employed for electrophoretic separation. However, in two of these isolates (C634 and C735), four chromosomes were visible on membrane transfers of pulsed-field gels after Southern hybridization between the chromosomal DNA and selected DNA probes. The probes included a conserved ribosomal gene and three previously described cDNAs isolated from C. immitis expression libraries. The L isolate was determined to have the same genome size as a typical four-chromosome isolate on the basis of microspectrophotometric comparison of fluorescence intensity of the ethidium bromide-stained nuclear DNA. The genome size of C. immitis determined by microspectrophotometry was approximately 28.2 +/- 2.6 Mb. The calculated genome size based on addition of the average molecular weights of chromosomal bands separated by contour-clamped homogeneous electric field gel electrophoresis was approximately equal to the estimate derived from the spectrophotometric analyses. This is the first report of the electrophoretic karyotype of C. immitis.
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