ArticleInfection and immunity1992
Interaction of the fish pathogen Aeromonas salmonicida with rainbow trout macrophages.
Article in Infection and immunity, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 49 citations in OpenAlex.
- Remedial Treatment of Corroded Iron Objects by EnvironmentalApplied and environmental microbiology · 2019Article
- Strong Genomic and Phenotypic Heterogeneity in theFrontiers in microbiology · 2017Article
- Transcriptome profiling reveals insight into distinct immune responses to Aeromonas salmonicida in gill of two rainbow trout strains.Marine biotechnology (New York, N.Y.) · 2014Article
- A comprehensive study on the role of the Yersinia pestis virulence markers in an animal model of pneumonic plague.Folia microbiologica · 2011Article
- Overcoming the heme paradox: heme toxicity and tolerance in bacterial pathogens.Infection and immunity · 2010Review
- Differential proteomic analysis of Clostridium perfringens ATCC13124; identification of dominant, surface and structure associated proteins.BMC microbiology · 2009Article
- Use of defined mutants to assess the role of the Campylobacter rectus S-layer in bacterium-epithelial cell interactions.Infection and immunity · 2000Article
- A Legionella pneumophila gene that promotes hemin binding.Infection and immunity · 1996Article
- Recombinant infectious hematopoietic necrosis virus and viral hemorrhagic septicemia virus glycoprotein epitopes expressed in Aeromonas salmonicida induce protective immunity in rainbow trout (Oncorhynchus mykiss).Applied and environmental microbiology · 1995Article
- A conserved Aeromonas salmonicida porin provides protective immunity to rainbow trout.Infection and immunity · 1995Article
- Physical and functional S-layer reconstitution in Aeromonas salmonicida.Journal of bacteriology · 1995Article
- Transcriptional analysis of the Aeromonas salmonicida S-layer protein gene vapA.Journal of bacteriology · 1993Article
- Aeromonas salmonicida grown in vivo.Infection and immunity · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A procedure was developed to culture rainbow trout macrophages (M phi) on supported glass coverslips. Using this method and a variety of well-characterized Aeromonas salmonicida strains with normal or altered cell surfaces, we investigated the role of this unusual bacterial surface in the bacterium-M phi interaction. An intact crystalline protein array, the A-layer, mediated adherence of A. salmonicida cells to M phi even in the absence of opsonins. In contrast, unopsonized cells of an A-layer-negative (A-) mutant with a smooth lipopolysaccharide (LPS) layer were unable to interact with M phi. However, this ability was recovered when the A-layer was reconstituted onto the smooth LPS surface of these A- LPS+ cells. Two A. salmonicida mutants possessing the A-layer in different disorganized states had a reduced ability to interact with M phi. A+ cells grown under calcium limitation produced A-layers locked into an alternative conformation which mediated the highest levels of M phi association in the absence of opsonins or any other surface coating. Coating A+ cells with hemin greatly increased their levels of M phi association, and bacterial cells grown on trout blood agar plates also had a dramatic increase in their ability to interact with M phi. Only A+ A. salmonicida cells were highly cytotoxic to trout M phi, especially after being coated with hemin, presumably due to a more focused targeting of the bacterial cell onto the M phi surface and/or into the intracellular regions of the M phi.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.