ArticleThe EMBO journal1992
Roles of PRP8 protein in the assembly of splicing complexes.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 91 citations in OpenAlex.
- LUBAC regulates ciliogenesis by promoting CP110 removal from the mother centriole.The Journal of cell biology · 2022Article
- A Snu114-GTP-Prp8 module forms a relay station for efficient splicing in yeast.Nucleic acids research · 2020Article
- Spt5 modulates cotranscriptional spliceosome assembly inRNA (New York, N.Y.) · 2019Article
- DHX15 promotes prostate cancer progression by stimulating Siah2-mediated ubiquitination of androgen receptor.Oncogene · 2018Article
- A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation.Nature structural & molecular biology · 2013Article
- The oligonucleotide binding (OB)-fold domain of KREPA4 is essential for stable incorporation into editosomes.PloS one · 2012Article
- Article
- Structure and function of an RNase H domain at the heart of the spliceosome.The EMBO journal · 2008Article
- Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing.RNA (New York, N.Y.) · 2007Article
- Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain.RNA (New York, N.Y.) · 2006Article
- Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p.RNA (New York, N.Y.) · 2006Article
- Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation.Genetics · 2005Article
- A complementation method for functional analysis of mammalian genes.Nucleic acids research · 2005Article
- Prp8 protein: at the heart of the spliceosome.RNA (New York, N.Y.) · 2005Review
- Article
- Evidence that the pre-mRNA splicing factor Clf1p plays a role in DNA replication in Saccharomyces cerevisiae.Genetics · 2002Article
- Evidence for a role of Sky1p-mediated phosphorylation in 3' splice site recognition involving both Prp8 and Prp17/Slu4.RNA (New York, N.Y.) · 2001Article
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- Article
- A tertiary interaction detected in a human U2-U6 snRNA complex assembled in vitro resembles a genetically proven interaction in yeast.RNA (New York, N.Y.) · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Three different approaches have been used to investigate the roles of the yeast U5 snRNP protein PRP8 in spliceosome assembly: genetic depletion of PRP8 protein in vivo, heat inactivation of temperature-sensitive prp8 protein in protoplasts and inhibition of PRP8 function with antibodies in vitro. In each case, U5 and U4/U6 snRNPs failed to assemble into the forming spliceosomes. In addition, extract prepared from PRP8-depleted cells and extract containing inactivated PRP8 protein had substantially reduced amounts of U4/U6.U5 triple snRNP complexes. Thus, functional PRP8 protein is required for the stable formation of U4/U6.U5 complexes without which spliceosomes fail to form. As spliceosome formation was also blocked by anti-PRP8 antibodies that apparently do not disrupt triple snRNPs, PRP8 protein may play a separate role in the assembly of triple snRNPs into spliceosomes. As a consequence of PRP8 depletion the levels of the U4, U5 and U6 snRNAs declined dramatically. We discuss this in the context of the known genetic interactions between PRP8 and putative RNA helicase (DEAD box protein) genes and propose that PRP8 protein plays a role in regulating dynamic RNA-RNA interactions in spliceosome assembly, possibly ensuring the correct directionality of these events.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.