ArticleThe Plant cell1992
A novel circadian phenotype based on firefly luciferase expression in transgenic plants.
Article in The Plant cell, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 152 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
152 citing papers in PubMed, 388 citations in OpenAlex.
- From phenomenon to mechanism in the circadian clock of Synechococcus elongatus.Npj biological timing and sleep · 2026Review
- SPIRAL: A versatile online single time-point circadian analysis platform for rice.Science advances · 2026Article
- Advances in monitoring mammalian circadian components and their rhythms using reporter systems.RSC chemical biology · 2026Review
- A PROMOTER::LUCIFERASE reporter system reveals key elements of the circadian regulation of Crassulacean acid metabolism (CAM) in Kalanchoë laxiflora Baker.The Plant journal : for cell and molecular biology · 2026Article
- Hyperspectral reporters for long-distance and wide-area detection of gene expression in living bacteria.Nature biotechnology · 2026Article
- Engineering glowing plants: recent progress and future directions for application-oriented design.Frontiers in bioengineering and biotechnology · 2026Review
- A CRISPR/Cas9-induced restoration of bioluminescence reporter system for single-cell gene expression analysis in plants.Scientific reports · 2025Article
- Model-based analysis of the circadian rhythm generation of bioluminescence reporter activity in duckweed.Plant biotechnology (Tokyo, Japan) · 2025Article
- Lost in translation: What we have learned from attributes that do not translate from Arabidopsis to other plants.The Plant cell · 2025Review
- Development of compounds for targeted degradation of mammalian cryptochrome proteins.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- The clock-associated LUX ARRHYTHMO regulates high-affinity nitrate transport in Arabidopsis roots.The Plant journal : for cell and molecular biology · 2024Article
- Reflections on Several Landmark Advances in Circadian Biology.Journal of circadian rhythms · 2024Review
- A Luciferase Imaging-Based Assay for Studying Temperature Compensation of the Circadian Clock.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Circadian regulation of metabolism across photosynthetic organisms.The Plant journal : for cell and molecular biology · 2023Review
- Inhibition of RNA degradation integrates the metabolic signals induced by osmotic stress into the Arabidopsis circadian system.Journal of experimental botany · 2023Article
- Assembly and operation of an imaging system for long-term monitoring of bioluminescent and fluorescent reporters in plants.Plant methods · 2023Article
- An auxin-mediated ultradian rhythm positively influences root regenerationFrontiers in plant science · 2023Article
- The circadian night depression of photosynthesis analyzed in a herb, Pulmonaria vallarsae. Day/night quantitative relationships.Photosynthesis research · 2022Article
- Cell-Type-Specific Circadian Bioluminescence Rhythms inJournal of biological rhythms · 2022Article
- Regeneration and Agrobacterium-mediated genetic transformation of twelveForestry research · 2022Article
92 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A 320-bp fragment of the Arabidopsis cab2 promoter is sufficient to mediate transcriptional regulation by both phytochrome and the circadian clock. We fused this promoter fragment to the firefly luciferase (Luc) gene to create a real-time reporter for regulated gene expression in intact plants. Cab2::Luc transcript accumulated in the expected patterns and luciferase activity was closely correlated to cab2::Luc mRNA abundance in both etiolated and green seedlings. The concentration of the bulk of luciferase protein did not reflect these patterns but maintained a relatively constant level, implying that a post-translational mechanism(s) leads to the high-amplitude regulation of luciferase activity. We used a low-light video imaging system to establish that luciferase bioluminescence in vivo accurately reports the temporal and spatial regulation of cab2 transcription in single seedlings. The unique qualities of the firefly luciferase system allowed us to monitor regulated gene expression in real time in individual multicellular organisms. This noninvasive marker for temporal regulation at the molecular level constitutes a circadian phenotype, which may be used to isolate mutants in the circadian clock.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.