ArticleThe Journal of cell biology1992
Cytoplasmic dynein participates in the centrosomal localization of the Golgi complex.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers.
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Who cites it
112 citing papers in PubMed, 283 citations in OpenAlex.
- Insights into Microtubule Nucleation at the Golgi Apparatus.Sub-cellular biochemistry · 2026Review
- Branching, crosslinking, and decentralization of microtubules accelerates intracellular assembly.Biophysical journal · 2025Article
- Cell cycle-regulated tug-of-war between microtubule motors positions major trafficking organelles.bioRxiv : the preprint server for biology · 2025Article
- The Chlamydia effector Dre1 binds dynactin to reposition host organelles during infection.Cell reports · 2025Article
- Huntingtin S421 phosphorylation increases kinesin and dynein engagement on early endosomes and lysosomes.Biophysical journal · 2023Article
- Schizophrenia-associated Mitotic Arrest Deficient-1 (MAD1) regulates the polarity of migrating neurons in the developing neocortex.Molecular psychiatry · 2023Article
- A pathogenic proteolysis-resistant huntingtin isoform induced by an antisense oligonucleotide maintains huntingtin function.JCI insight · 2022Article
- Nde1 and Ndel1: Outstanding Mysteries in Dynein-Mediated Transport.Frontiers in cell and developmental biology · 2022Review
- Doublecortin facilitates the elongation of the somatic Golgi apparatus into proximal dendrites.Molecular biology of the cell · 2021Article
- The functions of kinesin and kinesin-related proteins in eukaryotes.Cell adhesion & migration · 2020Review
- Growth cone-localized microtubule organizing center establishes microtubule orientation in dendrites.eLife · 2020Article
- The Generation of Dynein Networks by Multi-Layered Regulation and Their Implication in Cell Division.Frontiers in cell and developmental biology · 2020Review
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- Functions and dysfunctions of the mammalian centrosome in health, disorders, disease, and aging.Histochemistry and cell biology · 2018Review
- Fluorescence microscopy applied to intracellular transport by microtubule motors.Journal of biosciences · 2018Review
- Minus end-directed kinesin-14 KIFC1 regulates the positioning and architecture of the Golgi apparatus.Oncotarget · 2017Article
- Inositol Pyrophosphates: Energetic, Omnipresent and Versatile Signalling Molecules.Journal of the Indian Institute of Science · 2017Review
- Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport.The Biochemical journal · 2016Article
- Centrosome positioning in non-dividing cells.Protoplasma · 2016Review
52 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The localization of the Golgi complex depends upon the integrity of the microtubule apparatus. At interphase, the Golgi has a restricted pericentriolar localization. During mitosis, it fragments into small vesicles that are dispersed throughout the cytoplasm until telophase, when they again coalesce near the centrosome. These observations have suggested that the Golgi complex utilizes a dynein-like motor to mediate its transport from the cell periphery towards the minus ends of microtubules, located at the centrosome. We utilized semi-intact cells to study the interaction of the Golgi complex with the microtubule apparatus. We show here that Golgi complexes can enter semi-intact cells and associate stably with cytoplasmic constituents. Stable association, termed here "Golgi capture," requires ATP hydrolysis and intact microtubules, and occurs maximally at physiological temperature in the presence of added cytosolic proteins. Once translocated into the semi-intact cell cytoplasm, exogenous Golgi complexes display a distribution similar to endogenous Golgi complexes, near the microtubule-organizing center. The process of Golgi capture requires cytoplasmic tubulin, and is abolished if cytoplasmic dynein is immunodepleted from the cytosol. Cytoplasmic dynein, prepared from CHO cell cytosol, restores Golgi capture activity to reactions carried out with dynein immuno-depleted cytosol. These results indicate that cytoplasmic dynein can interact with isolated Golgi complexes, and participate in their accumulation near the centrosomes of semi-intact, recipient cells. Thus, cytoplasmic dynein appears to play a role in determining the subcellular localization of the Golgi complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.