ArticleProceedings of the National Academy of Sciences of the United States of America1992
Karyoplasmic interaction selection strategy: a general strategy to detect protein-protein interactions in mammalian cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 95 citations in OpenAlex.
- Article
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- Screening a cDNA library for protein-protein interactions directly in planta.The Plant cell · 2012Article
- The hepatitis C virus NS5A inhibitor (BMS-790052) alters the subcellular localization of the NS5A non-structural viral protein.Virology · 2011Article
- Single domain intracellular antibodies from diverse libraries: emphasizing dual functions of LMO2 protein interactions using a single VH domain.The Journal of biological chemistry · 2011Article
- Orphan nuclear receptors as targets for drug development.Pharmaceutical research · 2010Review
- The use of AlphaLISA technology to detect interaction between hepatitis C virus-encoded NS5A and cyclophilin A.Journal of virological methods · 2010Article
- Eos mediates Foxp3-dependent gene silencing in CD4+ regulatory T cells.Science (New York, N.Y.) · 2009Article
- De novo production of diverse intracellular antibody libraries.Nucleic acids research · 2003Article
- Computational approaches to protein-protein interaction.Journal of structural and functional genomics · 2003Article
- Envelope glycoprotein cytoplasmic domains from diverse lentiviruses interact with the prenylated Rab acceptor.Journal of virology · 2002Article
- Protein-protein interaction panel using mouse full-length cDNAs.Genome research · 2001Article
- Intracellular antibody-caspase-mediated cell killing: an approach for application in cancer therapy.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- A green fluorescent protein-reporter mammalian two-hybrid system with extrachromosomal maintenance of a prey expression plasmid: application to interaction screening.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Reporter gene vectors and assays.Molecular biotechnology · 1999Review
- Transcriptional activation by the product of open reading frame 50 of Kaposi's sarcoma-associated herpesvirus is required for lytic viral reactivation in B cells.Journal of virology · 1999Article
- Intranuclear localization of human papillomavirus 16 E7 during transformation and preferential binding of E7 to the Rb family member p130.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- c-Fos dimerization with c-Jun represses c-Jun enhancement of androgen receptor transactivation.Endocrine · 1998Article
- Transactivation domains facilitate promoter occupancy for the dioxin-inducible CYP1A1 gene in vivo.Molecular and cellular biology · 1997Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a strategy and reagents for study of protein-protein interactions in mammalian cells, termed the karyoplasmic interaction selection strategy (KISS). With this strategy, specific protein-protein interactions are identified by reconstitution of the functional activity of the yeast transcriptional activator GAL4 and the resultant transcription of a GAL4-regulated reporter gene. Reconstitution of GAL4 function results from specific interaction between two chimeric proteins: one contains the DNA-binding domain of GAL4; the other contains a transcriptional activation domain. Transcription of the reporter gene occurs if the two chimeric proteins can form a complex that reconstitutes the DNA-binding and transcriptional activation functions of GAL4. Using the KISS system, we demonstrate specific interactions for sequences from three different pairs of proteins that complex in the cytoplasm. In addition, we demonstrate that reporter genes encoding cell surface or drug-resistance markers can be specifically activated as a result of protein-protein interactions. With these selectable markers, the KISS system can be used to screen specialized cDNA libraries to identify novel protein interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.