ArticleThe Journal of clinical investigation1992
Surface expression of Fc gamma receptor III (CD16) on chemoattractant-stimulated neutrophils is determined by both surface shedding and translocation from intracellular storage compartments.
Article in The Journal of clinical investigation, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 111 citations in OpenAlex.
- Ketoprofen and prednisolone do not modulate neutrophil CR1, CR3 and Fc gamma RIII expression in healthy volunteers.British journal of clinical pharmacology · 1994Trial
- High-throughput quantitation of human neutrophil recruitment and functional responses in an air-blood barrier array.APL bioengineering · 2025Article
- Innate Immunity in Cystic Fibrosis: Varied Effects of CFTR Modulator Therapy on Cell-to-Cell Communication.International journal of molecular sciences · 2025Review
- Inflammation of the nasal mucosa is associated with susceptibility to experimental pneumococcal challenge in older adults.Mucosal immunology · 2024Article
- A subset of type-II collagen-binding antibodies prevents experimental arthritis by inhibiting FCGR3 signaling in neutrophils.Nature communications · 2023Article
- A cross-sectional study evidences regulations of leukocytes in the colostrum of mothers with obesity.BMC medicine · 2022Article
- The Low Expression of Fc-Gamma Receptor III (CD16) and High Expression of Fc-Gamma Receptor I (CD64) on Neutrophil Granulocytes Mark Severe COVID-19 Pneumonia.Diagnostics (Basel, Switzerland) · 2022Article
- Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2022Article
- Optimization of methods for the accurate characterization of whole blood neutrophils.Scientific reports · 2022Article
- Next Generation Natural Killer Cells for Cancer Immunotherapy.Frontiers in immunology · 2022Review
- Role of ADAM17 as a regulatory checkpoint of CD16A in NK cells and as a potential target for cancer immunotherapy.Journal of leukocyte biology · 2019Review
- Expression, Role, and Regulation of Neutrophil Fcγ Receptors.Frontiers in immunology · 2019Review
- Neutrophil plasticity enables the development of pathological microenvironments: implications for cystic fibrosis airway disease.Molecular and cellular pediatrics · 2016Review
- Neutrophil Fates in Bronchiectasis and Alpha-1 Antitrypsin Deficiency.Annals of the American Thoracic Society · 2016Article
- Mature cystic fibrosis airway neutrophils suppress T cell function: evidence for a role of arginase 1 but not programmed death-ligand 1.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Article
- Human peripheral neutrophils express functional IL-21 receptors.Inflammation · 2014Article
- ADAM17 cleaves CD16b (FcγRIIIb) in human neutrophils.Biochimica et biophysica acta · 2013Article
- The yin and yang of cystic fibrosis transmembrane conductance regulator function: implications for chronic lung disease.American journal of respiratory and critical care medicine · 2013Article
- Rapid decrease of CD16 (FcγRIII) expression on heat-shocked neutrophils and their recognition by macrophages.Journal of biomedicine & biotechnology · 2011Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Stimulation of neutrophils (PMN) with chemoattractants markedly increases surface expression of several membrane proteins, including the complement receptors, CR1 and CR3, by translocation from intracellular storage compartments to the cell surface. When we stimulated freshly-isolated PMN with FMLP, we observed little net change in surface Fc gamma receptor (R) III expression. However, if elastase was first used to cleave most (85-90%) of the Fc gamma R III from the PMN surface, subsequent treatment with FMLP induced a rapid renewal of surface Fc gamma R III, achieving levels of approximately 70% of that originally present on the cell surface after 15 min, suggesting translocation of intracellular receptors. This was confirmed by demonstrating concomitant depletion of greater than 80% of the intracellular Fc gamma R III. Studies of density gradient fractions of N2-cavitated PMN indicated at least two distinct intracellular membrane fractions that contain Fc gamma R III. Shedding of Fc gamma R III induced by FMLP was about half-maximal by 15 min and nearly complete by 60 min. Stoichiometric assessment of FMLP-induced changes in PMN surface and intracellular Fc gamma R III showed a marked depletion in intracellular Fc gamma R III, little net change in surface Fc gamma R III, and a large overall loss of total cell Fc gamma R III that could be attributed to shedding. We conclude that stimulation by chemoattractants causes a rapid translocation of intracellular Fc gamma R III to the PMN surface that is roughly balanced by the concomitant FMLP-induced shedding of this receptor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.