ArticleNucleic acids research1992
A new strategy useful for rapid identification of microsatellites from DNA libraries with large size inserts.
Article in Nucleic acids research, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 22 citations in OpenAlex.
- Identification and chromosomal localization of repeat sequences through BAC end sequence analysis in Korean cattle.Journal of genetics · 2005Article
- Exploiting dinucleotide microsatellites conserved among mammalian species.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- Uniparental isodisomy for paternal 7p and maternal 7q in a child with growth retardation.American journal of human genetics · 1994Article
- New nucleotide sequence data on the EMBL File Server.Nucleic acids research · 1992Article
- Mouse chromosome 19.Mammalian genome : official journal of the International Mammalian Genome Society · 1992Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microsatellites are new powerful polymorphic markers used for gene mapping. Their characterization requires that all the sequence surrounding the repeat be known in order to be able to design primers for PCR amplification. However, when using DNA libraries with large cloned inserts, this sequence characterization is not immediately practicable. In this paper, we describe a new strategy, based both on the use of a microsatellite specific probing and on the creation of nested deleted clones with the Exonuclease III, in order to position microsatellites in a range allowing direct sequencing. This method was applied to the screening of a mouse chromosome 19 DNA specific library. In this way, thirteen clones were identified by specific probing and seven were submitted to the nested deletion strategy. Five of them presented microsatellite sequences in specific deleted subclones which were selected and sequenced. Primers were designed for each of them and polymorphism between the genomes of several inbred strain of mouse have been determined. These microsatellites were mapped, three of them to chromosome 19 and two to chromosome 11.
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