ArticleProceedings of the National Academy of Sciences of the United States of America1992
Sensitivity to vanadate and isoforms of subunits A and B distinguish the osteoclast proton pump from other vacuolar H+ ATPases.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 152 citations in OpenAlex.
- HMedical molecular morphology · 2018Article
- Biochemical characteristics of the Ca2+ pumping ATPase in the peribacteroid membrane from broad bean root nodules.Protoplasma · 2013Article
- Osteoclasts: New Insights.Bone research · 2013Review
- V for victory--a V1-ATPase structure revealed.EMBO reports · 2009Article
- Matrix metalloproteinase-1 promotes breast cancer angiogenesis and osteolysis in a novel in vivo model.Breast cancer research and treatment · 2009Article
- Cytoplasmic terminus of vacuolar type proton pump accessory subunit Ac45 is required for proper interaction with V(0) domain subunits and efficient osteoclastic bone resorption.The Journal of biological chemistry · 2008Article
- Proton pump inhibitors and gastritis.Journal of clinical biochemistry and nutrition · 2008Article
- Conformation of a peptide encompassing the proton translocation channel of vacuolar H(+)-ATPase.Biophysical journal · 2007Article
- A novel inhibitor of vacuolar ATPase, FR167356, which can discriminate between osteoclast vacuolar ATPase and lysosomal vacuolar ATPase.British journal of pharmacology · 2004Article
- Osteoclast-independent bone resorption by fibroblast-like cells.Arthritis research & therapy · 2003Article
- Arg-735 of the 100-kDa subunit a of the yeast V-ATPase is essential for proton translocation.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Novel treatments for osteoporosis.The Journal of clinical investigation · 2000Article
- A selective inhibitor of the osteoclastic V-H(+)-ATPase prevents bone loss in both thyroparathyroidectomized and ovariectomized rats.The Journal of clinical investigation · 2000Article
- Breast cancer cells have a high capacity to acidify extracellular milieu by a dual mechanism.Clinical & experimental metastasis · 1997Article
- The vacuolar H+-ATPase: a universal proton pump of eukaryotes.The Biochemical journal · 1997Review
- Vacuolar H(+)-ATPase: from mammals to yeast and back.Experientia · 1996Review
- Molecular aspects of osteoclast function.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 1996Review
- Resorption-cycle-dependent polarization of mRNAs for different subunits of V-ATPase in bone-resorbing osteoclasts.Molecular biology of the cell · 1996Article
- Acid extrusion is induced by osteoclast attachment to bone. Inhibition by alendronate and calcitonin.The Journal of clinical investigation · 1995Article
- Alternative splicing generates a second isoform of the catalytic A subunit of the vacuolar H(+)-ATPase.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Analysis of proton (H+) transport by inside-out vesicles derived from highly purified chicken osteoclast (OC) membranes has revealed the presence of a newly discovered type of vacuolar H+ ATPase (V-ATPase). Unlike vesicles derived from any other cell type or organelle, H+ transport in OC-derived vesicles is sensitive to V-ATPase inhibitors (N-ethylmaleimide and Bafilomycin A1) and vanadate (IC50, 100 microM), an inhibitor previously found to affect only P-type ATPases. The OC H+ ATPase contains several V-like subunits (115, 39, and 16 kDa) but subunits A and B of the catalytic domain of the enzyme differ from that of other V-ATPases. In OCs, subunit A has a mass of 63 kDa instead of the 67-70 kDa expressed in monocytes, macrophages, and kidney microsomes, which contain a vanadate-insensitive H+ ATPase. Moreover, two types of 57- to 60-kDa B subunits are also found: one is expressed predominantly in OCs and the other is expressed in kidney microsomes. The OC H+ pump may therefore constitute a class of H+ ATPase with a unique pharmacology and specific isoforms of two subunits in the catalytic portion of the enzyme. This H+ ATPase is involved in resorption of bone and may be expressed in a cell-specific manner, thereby opening possibilities for therapeutic intervention.
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