ArticleProceedings of the National Academy of Sciences of the United States of America1992
Changing patterns in cytoskeletal mRNA expression and protein synthesis during murine erythropoiesis in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- High-throughput mechanical phenotyping and transcriptomics of single cells.Nature communications · 2024Article
- Reticulocyte Maturation.Membranes · 2022Review
- Transmission Electron Microscopy to Follow Ultrastructural Modifications of Erythroblasts UponFrontiers in physiology · 2021Article
- Severely impaired terminal erythroid differentiation as an independent prognostic marker in myelodysplastic syndromes.Blood advances · 2018Article
- Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.Scientific reports · 2017Article
- Primitive and definitive erythropoiesis in mammals.Frontiers in physiology · 2014Review
- Article
- Erythroblastic islands, terminal erythroid differentiation and reticulocyte maturation.International journal of hematology · 2011Review
- Disruption of spectrin-like cytoskeleton in differentiating keratinocytes by PKCδ activation is associated with phosphorylated adducin.PloS one · 2011Article
- Analysis of the kinetics of band 3 diffusion in human erythroblasts during assembly of the erythrocyte membrane skeleton.British journal of haematology · 2010Article
- Resolving the distinct stages in erythroid differentiation based on dynamic changes in membrane protein expression during erythropoiesis.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- An insulator with barrier-element activity promotes alpha-spectrin gene expression in erythroid cells.Blood · 2009Article
- Variegated expression from the murine band 3 (AE1) promoter in transgenic mice is associated with mRNA transcript initiation at upstream start sites and can be suppressed by the addition of the chicken beta-globin 5' HS4 insulator element.Molecular and cellular biology · 2003Article
- A nonsense mutation 1669Glu-->Ter within the regulatory domain of human erythroid ankyrin leads to a selective deficiency of the major ankyrin isoform (band 2.1) and a phenotype of autosomal dominant hereditary spherocytosis.The Journal of clinical investigation · 1995Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The major cytoskeletal proteins alpha-spectrin, beta-spectrin, and ankyrin are synthesized and assembled into a supportive membrane skeleton during erythroid differentiation. Information on the temporal appearance of mRNA and protein species is essential for understanding both the cytoskeletal assembly process and the function of various isoforms. We have isolated highly enriched populations of fetal erythroid cells at various stages of maturation. mRNAs for erythroid ankyrin, alpha-spectrin, and beta-spectrin were expressed at all stages but there were differences in transcript types and levels. The ratio of 9-kilobase (kb) to 7.5-kb erythroid ankyrin transcripts decreased markedly during differentiation, but there was no change in the ratio of the 10.1-kb and 9.3-kb erythroid beta-spectrin transcripts. The relative amounts of ankyrin, alpha-spectrin, and beta-spectrin mRNA increased during yolk sac cell differentiation, whereas only alpha-spectrin mRNA increased during differentiation of the fetal liver cells. The amounts of beta-spectrin mRNA exceeded the amounts of alpha-spectrin mRNA in the early precursors from both yolk sac and fetal liver; protein synthetic levels showed the same pattern. The 16-day fetal peripheral reticulocytes, on the other hand, had the adult mRNA and protein synthetic ratios with alpha/beta greater than 1. The data indicate that at least two mechanisms exist to meet changing erythroid membrane cytoskeletal requirements during development in utero: (i) stage-specific processing of the mRNA for the major cytoskeletal linker protein ankyrin and (ii) developmentally regulated alpha/beta-spectrin protein synthetic rates.
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