ArticleNucleic acids research1992
Insertion of non-intron sequence into maize introns interferes with splicing.
Article in Nucleic acids research, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Why Selection Might Be Stronger When Populations Are Small: Intron Size and Density Predict within and between-Species Usage of Exonic Splice Associated cis-Motifs.Molecular biology and evolution · 2015Article
- The maize brown midrib4 (bm4) gene encodes a functional folylpolyglutamate synthase.The Plant journal : for cell and molecular biology · 2015Article
- Alternative splicing in the coding region of Ppo-A1 directly influences the polyphenol oxidase activity in common wheat (Triticum aestivum L.).Functional & integrative genomics · 2011Article
- Pink-colored grape berry is the result of short insertion in intron of color regulatory gene.PloS one · 2011Article
- Addition of A- and U-rich sequence increases the splicing efficiency of a deleted form of a maize intron.Plant molecular biology · 1994Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Transposable element (TE) insertion into or near plant introns can cause intron skipping and alternative splicing events, resulting in reduced expression. To explore the impact of inserted sequences on splicing, we added non-intron sequence to two maize introns and tested these chimeric introns in a maize transient expression assay. Non-intron sequence inserted into Adh1-S intron 1 and actin intron 3 decreased expression from the luciferase reporter gene; the insertion sites tested were not in intron regions thought to be essential for splicing. Alternatively spliced mRNAs were not observed in transcripts derived from the insertion variants. In contrast, addition of an internal segment of an intron to Adh1-S intron 1 resulted in normal splice site selection and efficient processing. Because the normal intron sequence (including the conserved splice junctions) was retained in all constructs, we hypothesize that added non-intron sequence can interfere with intron recognition and/or splicing.
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Registered trials
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