ArticleNucleic acids research1992
Arbitrarily primed PCR fingerprinting of RNA.
Article in Nucleic acids research, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
93 citing papers in PubMed.
- Identification of Differentially Expressed Genes in Nocardia brasiliensis Induced by Progesterone and Dihydrotestosterone Using Differential Display PCR.Current microbiology · 2022Article
- Loss of Malat1 does not modify age- or diet-induced adipose tissue accretion and insulin resistance in mice.PloS one · 2018Article
- Identification and characterization of human MIBP1 gene in glioma cell differentiation.Journal of molecular neuroscience : MN · 2014Article
- SSHscreen and SSHdb, generic software for microarray based gene discovery: application to the stress response in cowpea.Plant methods · 2010Article
- Nitric oxide inhibits glomerular TGF-beta signaling via SMOC-1.Journal of the American Society of Nephrology : JASN · 2009Article
- Comparative transcriptome analyses of Pseudomonas aeruginosa.Human genomics · 2009Review
- Development and optimization of a fluorescent differential display PCR system for analyzing the stress response in Lactobacillus sakei strains.Nutrients · 2009Article
- Up-regulation and clinical relevance of novel helicase homologue DHX32 in colorectal cancer.Journal of experimental & clinical cancer research : CR · 2009Article
- Assessment of gene expression in many samples using vertical arrays.Nucleic acids research · 2008Article
- Identification of up-regulated genes in malignant glioma with subtraction hybridization: preliminary screening studies.Yonsei medical journal · 2008Article
- Advances in molecular marker techniques and their applications in plant sciences.Plant cell reports · 2008Review
- Identification of differentially expressed genes from Rhodothermus sp. XMH10 in response to low temperature using random arbitrarily primed PCR.Current microbiology · 2007Article
- Subtractive differential display: a modified differential display technique for isolating differentially expressed genes.Molecular biology reports · 2007Article
- Enhanced microarray performance using low complexity representations of the transcriptome.Nucleic acids research · 2005Article
- Methods for genome-wide analysis of gene expression changes in polyploids.Methods in enzymology · 2005Article
- Methods to find out the expression of activated genes.Reproductive biology and endocrinology : RB&E · 2004Review
- Amplification and overexpression of oncogene Mdm2 and orphan receptor gene Nr1h4 in immortal PRKDC knockout cells.Molecular biology reports · 2004Article
- Negative subtraction hybridization: an efficient method to isolate large numbers of condition-specific cDNAs.BMC genomics · 2004Article
- In vivo expression of the mannose-resistant fimbriae of Photorhabdus temperata K122 during insect infection.Journal of bacteriology · 2004Article
- Detection of differentially expressed genes in an isogenic breast metastasis model using RNA arbitrarily primed-polymerase chain reaction coupled with array hybridization (RAP-array).The American journal of pathology · 2004Article
33 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Fingerprinting of RNA populations was achieved using an arbitrarily selected primer at low stringency for first and second strand cDNA synthesis. PCR amplification was then used to amplify the products. The method required only a few nanograms of total RNA and was unaffected by low levels of genomic double stranded DNA contamination. A reproducible pattern of ten to twenty clearly visible PCR products was obtained from any one tissue. Differences in PCR fingerprints were detected for RNAs from the same tissue isolated from different mouse strains and for RNAs from different tissues from the same mouse. The strain-specific differences revealed are probably due to sequence polymorphisms and should be useful for genetic mapping of genes. The tissue-specific differences revealed may be useful for studying differential gene expression. Examples of tissue-specific differences were cloned. Differential expression was confirmed for these products by Northern analysis and DNA sequencing uncovered two new tissue-specific messages. The method should be applicable to the detection of differences between RNA populations in a wide variety of situations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.