ArticleJournal of clinical microbiology1992
Development of cDNA probes for typing group A bovine rotaviruses on the basis of VP4 specificity.
Article in Journal of clinical microbiology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Survey of equine rotaviruses shows conservation of one P genotype in background of two G genotypes.Archives of virology · 1996Article
- Serological and genomic characterisation of group A rotaviruses from lambs.Archives of virology · 1995Article
- Bovine rotavirus V1005 a P5, not a P12, type like all viruses in a German survey.Journal of clinical microbiology · 1994Article
- VP4 typing of bovine and porcine group A rotaviruses by PCR.Journal of clinical microbiology · 1994Article
- Relative frequencies of G (VP7) and P (VP4) serotypes determined by polymerase chain reaction assays among Japanese bovine rotaviruses isolated in cell culture.Journal of clinical microbiology · 1993Article
- Characterization of field strains of group A bovine rotaviruses by using polymerase chain reaction-generated G and P type-specific cDNA probes.Journal of clinical microbiology · 1993Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dot and Northern (RNA) blot hybridization assays were developed for the P typing of group A bovine rotaviruses (BRV) by using cDNA probes prepared from gene segment 4. The probes were prepared by polymerase chain reaction amplification of hyperdivergent regions (nucleotides 211 to 686) of BRV strain UK, IND, NCDV, and Cr VP4 cDNA by using specific oligonucleotide primers. The probes were P type specific (VP4) and exhibited little or no cross-reactivity with double-stranded RNA from heterologous rotavirus P types. Our studies indicate that at least three P types, as defined by polymerase chain reaction-derived VP4 gene probes from the UK, NCDV, and Cr strains, exist among the seven BRV isolates tested.
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