ArticlePflugers Archiv : European journal of physiology1992
Small-conductance Cl- channels in HT29 cells: activation by Ca2+, hypotonic cell swelling and 8-Br-cGMP.
Article in Pflugers Archiv : European journal of physiology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 65 citations in OpenAlex.
- TMEM16A in Cystic Fibrosis: Activating or Inhibiting?Frontiers in pharmacology · 2019Review
- Bestrophin-1 enables Ca2+-activated Cl- conductance in epithelia.The Journal of biological chemistry · 2009Article
- Hyposmotically activated chloride channels in cultured rabbit non-pigmented ciliary epithelial cells.The Journal of physiology · 1999Article
- A cyclic GMP-dependent housekeeping Cl- channel in rabbit gastric parietal cells activated by a vasodilator ecabapide.British journal of pharmacology · 1996Article
- Attenuation of stimulated Ca2+ influx in colonic epithelial (HT29) cells by cAMP.Pflugers Archiv : European journal of physiology · 1996Article
- Basolateral membrane chloride permeability of A6 cells: implication in cell volume regulation.Pflugers Archiv : European journal of physiology · 1995Article
- Ca2+ influx induced by store release and cytosolic Ca2+ chelation in Ht29 colonic carcinoma cells.Pflugers Archiv : European journal of physiology · 1995Article
- Volume regulation in a toad epithelial cell line: role of coactivation of K+ and Cl- channels.The Journal of physiology · 1995Article
- Volume-activated chloride currents are not correlated with P-glycoprotein expression.The Biochemical journal · 1995Article
- Molecular basis of epithelial Cl channels.The Journal of membrane biology · 1995Review
- A new class of inhibitors of cAMP-mediated Cl- secretion in rabbit colon, acting by the reduction of cAMP-activated K+ conductance.Pflugers Archiv : European journal of physiology · 1995Article
- Quantitative estimation of the area of luminal and basolateral membranes of rat parotid acinar cells: some physiological applications.Pflugers Archiv : European journal of physiology · 1994Article
- Model of bicarbonate secretion by resting frog stomach fundus mucosa. II. Role of the oxyntopeptic cells.Pflugers Archiv : European journal of physiology · 1994Article
- Volume-activated Cl- currents in different mammalian non-excitable cell types.Pflugers Archiv : European journal of physiology · 1994Article
- cAMP-dependent activation of ion conductances in bronchial epithelial cells.Pflugers Archiv : European journal of physiology · 1994Article
- Forskolin and PMA pretreatment of HT29 cells alters their chloride conductance induced by cAMP, Ca2+ and hypotonic cell swelling.Pflugers Archiv : European journal of physiology · 1994Article
- Permeation properties and modulation of volume-activated Cl(-)-currents in human endothelial cells.British journal of pharmacology · 1994Article
- The volume-activated chloride current in human endothelial cells depends on intracellular ATP.Pflugers Archiv : European journal of physiology · 1994Article
- Activation of a Cl- current by hypotonic volume increase in human endothelial cells.The Journal of general physiology · 1994Article
- Effect of ATP, carbachol and other agonists on intracellular calcium activity and membrane voltage of pancreatic ducts.Pflugers Archiv : European journal of physiology · 1994Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study demonstrates the activation of Cl- channels in HT29 cells by agonist (ATP, neurotensin, carbachol) increasing cytosolic Ca2+, by hypotonic cell swelling and by cGMP. Cell-attached nystatin patch-clamp (CAN) as well as slow and fast whole-cell recordings were used. The cell membrane potential was depolarized in a dose-dependent manner with half-maximal effects at 0.4 mumol/l for ATP, 60 pmol/l for neurotensin and 0.8 mumol/l for carbachol. The depolarization, which was caused by Cl- conductances increases, occurred within 1 s and was accompanied by a simultaneous and reversible increase of the input conductance of the cell-attached membrane from 295 +/- 32 pS to 1180 +/- 271 pS (ATP; 10 mumol/l, n = 21) and 192 +/- 37 pS to 443 +/- 128 pS (neurotensin; 1 nmol/l, n = 8). The effects of the agonists could be mimicked by ionomycin (0.2 mumol/l), suggesting that an increase in intracellular Ca2+ was responsible for the activation of Cl- channels. The depolarization was followed by a secondary hyperpolarization. Hypotonic cell swelling also depolarized the cells and induced an increase in the membrane conductance. With 120 mmol/l NaCl the depolarization was 10 +/- 0.8 mV and the cell-attached conductance increased from 228 +/- 29 pS to 410 +/- 65 (n = 26) pS. NaCl at 90 mmol/l and 72.5 mmol/l had even stronger effects. Comparable conductance increases were also obtained when the different agonists or hypotonic cell swelling were examined in whole cell experiments. 5-Nitro-2-(3-phenylpropylamino)-benzoate (1 mumol/l) did not prevent the effects of Ca(2+)-increasing hormones and of hypotonic solutions.(ABSTRACT TRUNCATED AT 250 WORDS)
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