ArticleProceedings of the National Academy of Sciences of the United States of America1992
Specificity of antisense oligonucleotides in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 251 citations in OpenAlex.
- Short 2'-O-methyl/LNA oligomers as highly-selective inhibitors of miRNA production in vitro and in vivo.Nucleic acids research · 2024Article
- The prorenin receptor and its soluble form contribute to lipid homeostasis.American journal of physiology. Endocrinology and metabolism · 2021Article
- Likelihood of Nonspecific Activity of Gapmer Antisense Oligonucleotides Is Associated with Relative Hybridization Free Energy.Nucleic acid therapeutics · 2020Article
- Hybridization-mediated off-target effects of splice-switching antisense oligonucleotides.Nucleic acids research · 2020Article
- Smurf1 Silencing Using a LNA-ASOs/Lipid Nanoparticle System to Promote Bone Regeneration.Stem cells translational medicine · 2019Article
- Identifying and avoiding off-target effects of RNase H-dependent antisense oligonucleotides in mice.Nucleic acids research · 2018Article
- RNase H sequence preferences influence antisense oligonucleotide efficiency.Nucleic acids research · 2017Article
- Managing the sequence-specificity of antisense oligonucleotides in drug discovery.Nucleic acids research · 2017Review
- Dissecting the target specificity of RNase H recruiting oligonucleotides using massively parallel reporter analysis of short RNA motifs.Nucleic acids research · 2015Article
- A short antisense oligonucleotide ameliorates symptoms of severe mouse models of spinal muscular atrophy.Molecular therapy. Nucleic acids · 2014Article
- The transcription factor CREB has no non-redundant functions in hepatic glucose metabolism in mice.Diabetologia · 2014Article
- Studies on the expression of sesquiterpene synthases using promoter-β-glucuronidase fusions in transgenic Artemisia annua L.PloS one · 2013Article
- Hydrolysis of bulged nucleotides in hybrids formed by RNA and imidazole-derivatized oligo-2'-O-methylribonucleotides.Nucleosides, nucleotides & nucleic acids · 2011Article
- Variables and strategies in development of therapeutic post-transcriptional gene silencing agents.Journal of ophthalmology · 2011Article
- The use of antisense oligonucleotides in Xenopus oocytes.Methods (San Diego, Calif.) · 2010Review
- Regulation of apoptosis by fau revealed by functional expression cloning and antisense expression.Oncogene · 2004Article
- Expanding the design horizon of antisense oligonucleotides with alpha-L-LNA.Nucleic acids research · 2003Article
- In vivo evidence that defects in the transcriptional elongation factors RPB2, TFIIS, and SPT5 enhance upstream poly(A) site utilization.Molecular and cellular biology · 2003Article
- Molecular recognition properties of IGS-mediated reactions catalyzed by a Pneumocystis carinii group I intron.Nucleic acids research · 2003Article
- Prioritized selection of oligodeoxyribonucleotide probes for efficient hybridization to RNA transcripts.Nucleic acids research · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antisense oligonucleotides are widely used as inhibitors of gene expression in cultured cells and have been proposed as potential therapeutic agents, but it is not known to what extent they are specific for their intended target RNAs. Statistical considerations indicate that if oligonucleotides can form hybrids with mRNA molecules in vivo by means of short or imperfect regions of complementarity, then the specificity of oligonucleotides as antisense reagents will be greatly compromised. We have used Xenopus oocytes as a model system in which to investigate the potential specificity of antisense oligonucleotides in vivo. We injected perfect and partially matched antisense oligonucleotides into oocytes and measured the resulting degradation of the target RNA in each case. On the basis of the extent to which antisense oligonucleotides can cause cleavage of RNAs at imperfectly matched target sites, we conclude that in this system it is probably not possible to obtain specific cleavage of an intended target RNA without also causing at least the partial destruction of many nontargeted RNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.