ArticleThe Journal of cell biology1992
Identification of a peptide sequence involved in homophilic binding in the neural cell adhesion molecule NCAM.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 146 citations in OpenAlex.
- Down syndrome cell adhesion molecule like-1 (DSCAML1) links the GABA system and seizure susceptibility.Acta neuropathologica communications · 2020Article
- Characterization of V-set and immunoglobulin domain containing 1 exerting a tumor suppressor function in gastric, lung, and esophageal cancer cells.Cancer science · 2017Article
- Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis.Experimental cell research · 2015Article
- Cell adhesion molecules in metastatic neuroblastoma models.Clinical & experimental metastasis · 2014Article
- Chondroitin sulfate proteoglycans: structure-function relationship with implication in neural development and brain disorders.BioMed research international · 2014Review
- Single-molecule force spectroscopy of the Aplysia cell adhesion molecule reveals two homophilic bonds.Biophysical journal · 2012Article
- Pharmacology of cell adhesion molecules of the nervous system.Current neuropharmacology · 2007Article
- Specific inhibition of GPI-anchored protein function by homing and self-association of specific GPI anchors.The Journal of cell biology · 2006Article
- Multiple-bond kinetics from single-molecule pulling experiments: evidence for multiple NCAM bonds.Biophysical journal · 2005Article
- Mechanism of homophilic adhesion by the neural cell adhesion molecule: use of multiple domains and flexibility.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Intracellular signaling by the neural cell adhesion molecule.Neurochemical research · 2003Review
- Genomic organization of a novel glycosylphosphatidylinositol MAM gene expressed in human tissues and tumors.Oncogene · 2002Article
- The specificity for the differentiation blocking activity of carcinoembryonic antigen resides in its glycophosphatidyl-inositol anchor.The Journal of cell biology · 2000Article
- Neural cell adhesion molecule (N-CAM) domains and intracellular signaling pathways involved in the inhibition of astrocyte proliferation.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Homophilic adhesion mediated by the neural cell adhesion molecule involves multiple immunoglobulin domains.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Migration of monocytes across endothelium and passage through extracellular matrix involve separate molecular domains of PECAM-1.The Journal of experimental medicine · 1995Article
- Functional analysis of posttranslational cleavage products of the neuron-glia cell adhesion molecule, Ng-CAM.The Journal of cell biology · 1995Article
- Neural cell adhesion molecule (NCAM) as a quantitative marker in synaptic remodeling.Neurochemical research · 1995Review
- The neural cell adhesion molecule (N-CAM) inhibits proliferation in primary cultures of rat astrocytes.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Expression of the Neural Cell Adhesion Molecule NCAM by Peptide- and Steroid-Producing Endocrine Cells and Tumors: Alternatively Spliced Forms and Polysialylation.Endocrine pathology · 1995Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The neural cell adhesion molecule NCAM is capable of mediating cell-cell adhesion via homophilic interactions. In this study, three strategies have been combined to identify regions of NCAM that participate directly in NCAM-NCAM binding: analysis of domain deletion mutations, mapping of epitopes of monoclonal antibodies, and use of synthetic peptides to inhibit NCAM activity. Studies on L cells transfected with NCAM mutant cDNAs using cell aggregation and NCAM-covasphere binding assays indicate that the third immunoglobulin-like domain is involved in homophilic binding. The epitopes of four monoclonal antibodies that have been previously shown to affect cell-cell adhesion mediated by NCAM were also mapped to domain 3. Overlapping hexapeptides were synthesized on plastic pins and assayed for binding with these monoclonal antibodies. One of them (PP) reacted specifically with the sequence KYSFNY. Synthetic oligopeptides containing the PP epitope were potent and specific inhibitors of NCAM binding activity. A substratum containing immobilized peptide conjugates also exhibited adhesiveness for neural retinal cells. Cell attachment was specifically inhibited by peptides that contained the PP-epitope and by anti-NCAM univalent antibodies. The shortest active peptide has the sequence KYSFNYDGSE, suggesting that this site is directly involved in NCAM homophilic interaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.