ArticleThe EMBO journal1992
Two different protein kinases act on a different time schedule as glial filament kinases during mitosis.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 100 citations in OpenAlex.
- Article
- Intermediate filaments and IF-associated proteins: from cell architecture to cell proliferation.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2019Review
- Identification of non-Ser/Thr-Pro consensus motifs for Cdk1 and their roles in mitotic regulation of C2H2 zinc finger proteins and Ect2.Scientific reports · 2015Article
- Signaling mechanisms and disrupted cytoskeleton in the diphenyl ditelluride neurotoxicity.Oxidative medicine and cellular longevity · 2014Review
- Defect of mitotic vimentin phosphorylation causes microophthalmia and cataract via aneuploidy and senescence in lens epithelial cells.The Journal of biological chemistry · 2013Article
- Phosphorylation of GFAP is associated with injury in the neonatal pig hypoxic-ischemic brain.Neurochemical research · 2012Article
- Phosphoproteomic analysis reveals site-specific changes in GFAP and NDRG2 phosphorylation in frontotemporal lobar degeneration.Journal of proteome research · 2010Article
- Expression of tryptophan 2,3-dioxygenase in mature granule cells of the adult mouse dentate gyrus.Molecular brain · 2010Article
- Immunolocalization of fascin, an actin-bundling protein and glial fibrillary acidic protein in human astrocytoma cells.Brain pathology (Zurich, Switzerland) · 2010Article
- Role of phosphorylation on the structural dynamics and function of types III and IV intermediate filaments.Experimental cell research · 2007Review
- Regulatory mechanisms and functions of intermediate filaments: a study using site- and phosphorylation state-specific antibodies.Cancer science · 2006Review
- Functional significance of the specific sites phosphorylated in desmin at cleavage furrow: Aurora-B may phosphorylate and regulate type III intermediate filaments during cytokinesis coordinatedly with Rho-kinase.Molecular biology of the cell · 2003Article
- Article
- Article
- Roles of Rho-associated kinase in cytokinesis; mutations in Rho-associated kinase phosphorylation sites impair cytokinetic segregation of glial filaments.The Journal of cell biology · 1998Article
- Adducin is an in vivo substrate for protein kinase C: phosphorylation in the MARCKS-related domain inhibits activity in promoting spectrin-actin complexes and occurs in many cells, including dendritic spines of neurons.The Journal of cell biology · 1998Article
- Intermediate filaments as dynamic structures.Cancer metastasis reviews · 1996Review
- Implications of intermediate filament protein phosphorylation.Cancer metastasis reviews · 1996Review
- Visualization of protein kinase activities in single cells by antibodies against phosphorylated vimentin and GFAP.Neurochemical research · 1996Review
- Disrupted glial fibrillary acidic protein network in astrocytes from vimentin knockout mice.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glial fibrillary acidic protein (GFAP) is a component of glial filaments specific to astroglia. We now report the spatial and temporal distributions of four phosphorylated sites in the GFAP molecule during mitosis of astroglial cells, determined by antibodies which can distinguish phosphorylated epitopes from non-phosphorylated-epitopes. Immunofluorescence microscopy showed that the Ser8 residues in the entire cytoplasmic glial filament system are initially phosphorylated when the cells enter mitosis. In cytokinesis, the phosphoSer8 residues become dephosphorylated, whereas Thr7, Ser13 and Ser34 in glial filaments at the cleavage furrow become the preferred sites of phosphorylation. The cdc2 kinase purified from mitotic cells can phosphorylate GFAP at Ser8 but not at Thr7, Ser13 or Ser34, in vitro. These results suggest that cdc2 kinase acts as a glial filament kinase only at the G2-M phase transition while other glial filament kinases are probably activated at the cleavage furrow before final separation of the daughter cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.