ArticleThe Journal of cell biology1992
Biochemical and immunological characterization of p190-calmodulin complex from vertebrate brain: a novel calmodulin-binding myosin.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 112 citations in OpenAlex.
- Discovery of the first unconventional myosin:Frontiers in physiology · 2023Review
- RACK1 plays a critical role in mast cell secretion and Ca2+ mobilization by modulating F-actin dynamics.Journal of cell science · 2021Article
- The Role of Calmodulin vs. Synaptotagmin in Exocytosis.Frontiers in molecular neuroscience · 2021Review
- Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2013Article
- The streptozotocin-induced rat model of diabetes mellitus evidences significant reduction of myosin-Va expression in the brain.Metabolic brain disease · 2011Article
- Myosin-Va-interacting protein, RILPL2, controls cell shape and neuronal morphogenesis via Rac signaling.Journal of cell science · 2009Article
- Alternative splicing in class V myosins determines association with Rab10.The Journal of biological chemistry · 2009Article
- A molecular dynamics study and free energy analysis of complexes between the Mlc1p protein and two IQ motif peptides.Biophysical journal · 2006Article
- Griscelli syndrome: characterization of a new mutation and rescue of T-cytotoxic activity by retroviral transfer of RAB27A gene.Journal of clinical immunology · 2004Article
- Targeting of calcium/calmodulin-dependent protein kinase II.The Biochemical journal · 2004Review
- Calpain regulates enterocyte brush border actin assembly and pathogenic Escherichia coli-mediated effacement.The Journal of biological chemistry · 2003Article
- Myosin Va binding to neurofilaments is essential for correct myosin Va distribution and transport and neurofilament density.The Journal of cell biology · 2002Article
- Myosin Va bound to phagosomes binds to F-actin and delays microtubule-dependent motility.Molecular biology of the cell · 2001Article
- The kinetic mechanism of myosin V.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Inhibition of dendrite formation in mouse melanocytes transiently transfected with antisense DNA to myosin Va.Journal of anatomy · 1999Article
- Rho3 of Saccharomyces cerevisiae, which regulates the actin cytoskeleton and exocytosis, is a GTPase which interacts with Myo2 and Exo70.Molecular and cellular biology · 1999Article
- Identification of functional connections between calmodulin and the yeast actin cytoskeleton.Genetics · 1998Article
- Mlc1p is a light chain for the unconventional myosin Myo2p in Saccharomyces cerevisiae.The Journal of cell biology · 1998Article
- Brain myosin V is a synaptic vesicle-associated motor protein: evidence for a Ca2+-dependent interaction with the synaptobrevin-synaptophysin complex.The Journal of cell biology · 1997Article
- Cultured melanocytes from dilute mutant mice exhibit dendritic morphology and altered melanosome distribution.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
We have recently identified a novel 190-kD calmodulin-binding protein (p190) associated with the actin-based cytoskeleton from mammalian brain (Larson, R. E., D. E. Pitta, and J. A. Ferro. 1988. Braz. J. Med. Biol. Res. 21:213-217; Larson, R. E., F. S. Espindola, and E. M. Espreafico. 1990. J. Neurochem. 54:1288-1294). These studies indicated that p190 is a phosphoprotein substrate for calmodulin-dependent kinase II and has calcium- and calmodulin-stimulated MgATPase activity. We now have biochemical and immunological evidence that this protein is a novel calmodulin-binding myosin whose properties include (a) Ca2+ dependent action activation of its Mg-ATPase activity, which seems to be mediated by Ca2+ binding directly to calmodulin(s) associated with p190 (maximal activation by actin requires the presence of Ca2+ and is further augmented by addition of exogenous calmodulin); (b) ATP-sensitive cross-linking of skeletal muscle F-actin, as demonstrated by the low-speed actin sedimentation assay; and (c) cross-reactivity with mAbs specific for epitopes in the head of brush border myosin I. We also show that p190 has properties distinct from conventional brain myosin II and brush border myosin I, including (a) separation of p190 from brain myosin II by gel filtration on a Sephacryl S-500 column; (b) lack by p190 of K(+)-stimulated EDTA ATPase activity characteristic of most myosins; (c) lack of immunological cross-reactivity of polyclonal antibodies which recognize p190 and brain myosin II, respectively; (d) lack of immunological recognition of p190 by mAbs against an epitope in the tail region of brush border myosin I; and (e) distinctive proteolytic susceptibility to calpain. A survey of rat tissues by immunoblotting indicated that p190 is expressed predominantly in the adult forebrain and cerebellum, and could be detected in embryos 11 d post coitus. Immunocytochemical studies showed p190 to be present in the perikarya and dendritic extensions of Purkinje cells of the cerebellum.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.