ArticleThe EMBO journal1992
Regulation of CFTR expression and function during differentiation of intestinal epithelial cells.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 76 citations in OpenAlex.
- Allosteric inhibition of CFTR gating by CFTRinh-172 binding in the pore.Nature communications · 2024Article
- Functional Consequences of CFTR Interactions in Cystic Fibrosis.International journal of molecular sciences · 2024Review
- What Role Does CFTR Play in Development, Differentiation, Regeneration and Cancer?International journal of molecular sciences · 2020Review
- Expression, Localization and Functional Activity of the Major Na⁺/H⁺ Exchange Isoforms Expressed in the Intestinal Cell Line Caco-2BBe.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2019Article
- Prolonged co-treatment with HGF sustains epithelial integrity and improves pharmacological rescue of Phe508del-CFTR.Scientific reports · 2018Article
- Article
- T84 monolayers are superior to Caco-2 as a model system of colonocytes.Histochemistry and cell biology · 2017Article
- Recent Progress in CFTR Interactome Mapping and Its Importance for Cystic Fibrosis.Frontiers in pharmacology · 2017Review
- Cytokine-Regulation of NaFrontiers in immunology · 2017Review
- Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein.Nature biotechnology · 2012Article
- Article
- Cell-type-specific long-range looping interactions identify distant regulatory elements of the CFTR gene.Nucleic acids research · 2010Article
- Cell culture models demonstrate that CFTR dysfunction leads to defective fatty acid composition and metabolism.Journal of lipid research · 2008Article
- Tetramethylpyrazine stimulates cystic fibrosis transmembrane conductance regulator-mediated anion secretion in distal colon of rodents.World journal of gastroenterology · 2005Article
- Differential Cl- and HCO3- mediated anion secretion by different colonic cell types in response to tetromethylpyrazine.World journal of gastroenterology · 2004Article
- CFTR: covalent modification of cysteine-substituted channels expressed in Xenopus oocytes shows that activation is due to the opening of channels resident in the plasma membrane.The Journal of general physiology · 2001Article
- Biosynthesis of mucin type O-glycans: lack of correlation between glycosyltransferase and sulfotransferase activities and CFTR expression.Glycoconjugate journal · 2001Article
- Inhibition of volume-regulated anion channels by expression of the cystic fibrosis transmembrane conductance regulator.The Journal of physiology · 1999Article
- Na-K-2Cl cotransporter gene expression and function during enterocyte differentiation. Modulation of Cl- secretory capacity by butyrate.The Journal of clinical investigation · 1998Article
- A dicistronic construct allows easy detection of human CFTR expression from YAC DNA in human cells.Nucleic acids research · 1997Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CFTR, the protein defective in cystic fibrosis is regulated during differentiation of intestinal epithelial cells. The undifferentiated cells (Caco-2 and HT-29) show a lower level of CFTR mRNA, while a 10-fold increase is seen in differentiated cells. These differences correlate well with those of other intestinal-specific genes, including sucrase-isomaltase, villin and alpha 1-antitrypsin, indicating that the regulation is cell specific. In Caco-2 cells the increase in CFTR mRNA cannot be accounted for by increased transcription of the gene. These data indicate that CFTR mRNA stabilizing factor(s) might be present in differentiated cells. The higher levels of CFTR mRNA in differentiated cells are accompanied by decreased protein levels, indicating, as well, involvement of translational control in the regulation of CFTR in these cells. Finally, fully differentiated cells show lowered levels of cyclic AMP-activated C1- transport, the characteristic function of CFTR. Thus, CFTR function in differentiated cells is modulated by a complex interaction of regulatory elements. Caco-2 and HT-29 cells provide a suitable in vitro system in which to study the mechanism of regulation of CFTR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.