ArticleThe Biochemical journal1992
Isolation and characterization of a membrane protein from rat erythrocytes which inhibits lysis by the membrane attack complex of rat complement.
Article in The Biochemical journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 86 citations in OpenAlex.
- Article
- The crystal structure of cobra venom factor, a cofactor for C3- and C5-convertase CVFBb.Structure (London, England : 1993) · 2009Article
- A protein toxin from the sea anemone Phyllodiscus semoni targets the kidney and causes a severe renal injury with predominant glomerular endothelial damage.The American journal of pathology · 2007Article
- Characterization of the mouse analogues of CD59 using novel monoclonal antibodies: tissue distribution and functional comparison.Immunology · 2003Article
- Blockage of complement regulators in the conjunctiva and within the eye leads to massive inflammation and iritis.Immunology · 2001Article
- Interaction between host complement and mosquito-midgut-stage Plasmodium berghei.Infection and immunity · 2001Article
- GluR3 autoantibodies destroy neural cells in a complement-dependent manner modulated by complement regulatory proteins.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Chronic low level complement activation within the eye is controlled by intraocular complement regulatory proteins.Investigative ophthalmology & visual science · 2000Article
- High-resolution FRET microscopy of cholera toxin B-subunit and GPI-anchored proteins in cell plasma membranes.Molecular biology of the cell · 2000Article
- Production and functional characterization of a soluble recombinant form of mouse CD59.Immunology · 2000Article
- Comparison of the suppressive effects of soluble CR1 and C5a receptor antagonist in acute arthritis induced in rats by blocking of CD59.Clinical and experimental immunology · 2000Article
- Production and functional analysis of rat CD59 and chimeric CD59-Crry as active soluble proteins in Pichia pastoris.Immunology · 2000Article
- Article
- Protection of human breast cancer cells from complement-mediated lysis by expression of heterologous CD59.Clinical and experimental immunology · 1999Article
- Characterization in vitro and in vivo of the pig analogue of human CD59 using new monoclonal antibodies.Immunology · 1998Article
- Membrane attack complex of complement and 20 kDa homologous restriction factor (CD59) in myocardial infarction.Virchows Archiv : an international journal of pathology · 1997Article
- Expression of rat CD59: functional analysis confirms lack of species selectivity and reveals that glycosylation is not required for function.Immunology · 1997Article
- Expression and function of membrane regulators of complement on rat astrocytes in culture.Immunology · 1996Article
- Tubulointerstitial injury induced in rats by a monoclonal antibody that inhibits function of a membrane inhibitor of complement.The Journal of clinical investigation · 1995Article
- Molecular cloning of the rat analogue of human CD59: structural comparison with human CD59 and identification of a putative active site.The Biochemical journal · 1994Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The membrane attack complex (MAC) of complement in humans is regulated by several membrane-bound proteins; however, no such proteins have so far been described in other species. Here we report the isolation and characterization of a rat erythrocyte membrane glycoprotein of molecular mass 21 kDa which inserts into cell membranes and is a potent inhibitor of the rat MAC. This protein, here called rat inhibitory protein (RIP), was first partially purified by column chromatography from a butanol extract of rat erythrocyte membranes. Monoclonal antibodies (Mabs) were raised against RIP and used for its affinity purification. Affinity-purified RIP was shown to inhibit in a dose-dependent manner the cobra venom factor (CVF)-mediated 'reactive' lysis of guinea pig erythrocytes by rat complement. Conversely, the anti-RIP MAbs 6D1 and TH9 were shown to markedly enhance the CVF-mediated lysis of rat erythrocytes by rat complement. RIP acted late in the assembly of the MAC (at or after the C5b-8 stage) and was releasable from the membranes of rat erythrocytes by phosphatidylinositol-specific phospholipase C. These features, together with its size, deglycosylation pattern and N-terminal amino acid sequence, lead us to conclude that RIP is the rat homologue of the human MAC-inhibitory protein CD59 antigen.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.