ArticleThe Journal of cell biology1992
Slow axonal transport mechanisms move neurofilaments relentlessly in mouse optic axons.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 84 citations in OpenAlex.
- The role of neurofilament transport in the radial growth of myelinated axons.Molecular biology of the cell · 2023Article
- Article
- Local Acceleration of Neurofilament Transport at Nodes of Ranvier.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Giant axonal neuropathy-associated gigaxonin mutations impair intermediate filament protein degradation.The Journal of clinical investigation · 2013Article
- A critical reevaluation of the stationary axonal cytoskeleton hypothesis.Cytoskeleton (Hoboken, N.J.) · 2013Review
- Neurofilaments at a glance.Journal of cell science · 2012Review
- Neurofilaments are flexible polymers that often fold and unfold, but they move in a fully extended configuration.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Interaction with a kinesin-2 tail propels choline acetyltransferase flow towards synapse.Traffic (Copenhagen, Denmark) · 2012Article
- Axonal transport of neurofilaments: a single population of intermittently moving polymers.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Neurofilaments form a highly stable stationary cytoskeleton after reaching a critical level in axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Myosin Va increases the efficiency of neurofilament transport by decreasing the duration of long-term pauses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Conditional NF-L transgene expression in mice for in vivo analysis of turnover and transport rate of neurofilaments.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Neurofilaments switch between distinct mobile and stationary states during their transport along axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- A dynamical system model of neurofilament transport in axons.Journal of theoretical biology · 2005Article
- Stochastic simulation of neurofilament transport in axons: the "stop-and-go" hypothesis.Molecular biology of the cell · 2005Article
- Arrival, reversal, and departure of neurofilaments at the tips of growing axons.Molecular biology of the cell · 2004Article
- Abnormal neurofilament transport caused by targeted disruption of neuronal kinesin heavy chain KIF5A.The Journal of cell biology · 2003Article
- Neurofilaments consist of distinct populations that can be distinguished by C-terminal phosphorylation, bundling, and axonal transport rate in growing axonal neurites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Neurofilaments are transported rapidly but intermittently in axons: implications for slow axonal transport.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Glutamate slows axonal transport of neurofilaments in transfected neurons.The Journal of cell biology · 2000Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pulse-labeling studies of slow axonal transport in many kinds of axons (spinal motor, sensory ganglion, oculomotor, hypoglossal, and olfactory) have led to the inference that axonal transport mechanisms move neurofilaments (NFs) unidirectionally as a single continuous kinetic population with a diversity of individual transport rates. One study in mouse optic axons (Nixon, R. A., and K. B. Logvinenko. 1986. J. Cell Biol. 102:647-659) has given rise to the different suggestion that a significant and distinct population of NFs may be entirely stationary within axons. In mouse optic axons, there are relatively few NFs and the NF proteins are more lightly labeled than other slowly transported slow component b (SCb) proteins (which, however, move faster than the NFs); thus, in mouse optic axons, the radiolabel of some of these faster-moving SCb proteins may confuse NF protein analyses that use one dimensional (1-D) SDS-PAGE, which separates proteins by size only. To test this possibility, we used a 2-mm "window" (at 3-5 mm from the posterior of the eye) to compare NF kinetics obtained by 1-D SDS-PAGE and by the higher resolution two-dimensional (2-D) isoelectric focusing/SDS-PAGE, which separates proteins both by their net charge and by their size. We found that 1-D SDS-PAGE is insufficient for definitive NF kinetics in the mouse optic system. By contrast, 2-D SDS-PAGE provides essentially pure NF kinetics, and these indicate that in the NF-poor mouse optic axons, most NFs advance as they do in other, NF-rich axons. In mice, greater than 97% of the radiolabeled NFs were distributed in a unimodal wave that moved at a continuum of rates, between 3.0 and 0.3 mm/d, and less than 0.1% of the NF population traveled at the very slowest rates of less than 0.005 mm/d. These results are inconsistent with the proposal (Nixon and Logvinenko, 1986) that 32% of the transported NFs remain within optic axons in an entirely stationary state. As has been found in other axons, the axonal transport system of mouse optic axons moves NFs and other cytoskeletal elements relentlessly from the cell body to the axon tip.
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