ArticleCalcified tissue international1992
Expression of collagen, osteocalcin, and bone alkaline phosphatase in a mineralizing rat osteoblastic cell culture.
Article in Calcified tissue international, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 106 citations in OpenAlex.
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- A new method to investigate how mechanical loading of osteocytes controls osteoblasts.Frontiers in endocrinology · 2014Article
- Hedgehog signaling in tumor cells facilitates osteoblast-enhanced osteolytic metastases.PloS one · 2012Article
- Biomineralization and matrix vesicles in biology and pathology.Seminars in immunopathology · 2011Review
- rBMP represses Wnt signaling and influences skeletal progenitor cell fate specification during bone repair.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2010Article
- Dental follicle progenitor cell heterogeneity in the developing mouse periodontium.Stem cells and development · 2006Article
- Multilineage differentiation of adipose-derived stromal cells from GFP transgenic mice.Molecular and cellular biochemistry · 2006Article
- Intermittent compressive load stimulates osteogenesis and improves osteocyte viability in bones cultured "in vitro".Clinical rheumatology · 1996Article
- ALP induction by beta-glycerophosphate during the non-mineralization phase in vitro.Journal of Tongji Medical University = Tong ji yi ke da xue xue bao · 1996Article
- Characterization of the phosphatidylinositol-specific phospholipase C-released form of rat osseous plate alkaline phosphatase and its possible significance on endochondral ossification.Molecular and cellular biochemistry · 1995Article
- Bone formation in vitro and in nude mice by human osteosarcoma cells.Virchows Archiv : an international journal of pathology · 1995Article
- Bioactive glass-ceramic containing crystalline apatite and wollastonite initiates biomineralization in bone cell cultures.Calcified tissue international · 1994Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rat calvaria bone cells isolated by collagenase digestion form a bone-like matrix which mineralizes in vitro in the presence of beta-glycerophosphate, in less than 2 weeks. The purpose of this work was to investigate, in this mineralizing rat osteoblastic cell culture, the synthesis of collagen, osteocalcin, and bone alkaline phosphatase (ALP). The results obtained indicate (1) After 15 days in culture, the extracellular-matrix contains collagen type I, V, and to some extent type III. Metabolic labeling at day 14, during the phase of nodules mineralization as well as new nodules formation, shows that collagen types I and type V are synthesized; (2) During the phase of cell growth, no osteocalcin could be detected in the medium, however, at the point of nodule formation, the osteocalcin level reached values of 3.55 +/- 1.39 ng/ml, followed by a 30-fold increase after nodules became mineralized. At day 14, after metabolic labeling, de novo synthesized osteocalcin was chromatographed on an immunoadsorbing column. With urea-SDS PAGE the apparent molecular weight was determined to be 9,000 daltons. (3) Specific activity of ALP was found to be 10 nmol/min/mg of proteins at cell confluence. At day 15, when nodules are mineralized, this activity was increased by 40-fold. The Michaelis constant was 1.58 10(-3) M/L. ALP was inhibited by L-homoarginine and levamisole but not by L-phenylalanine. ALP was shown to be heat sensitive at 56 degrees C with two slopes of inhibition.(ABSTRACT TRUNCATED AT 250 WORDS)
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