ArticleMolecular and cellular biology1992
Altered chromosome 6 in immortal human fibroblasts.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 60 citations in OpenAlex.
- Current treatment and novel insights regarding ROS1-targeted therapy in malignant tumors.Cancer medicine · 2024Review
- Establishment of a conditionally immortalized mouse optic nerve astrocyte line.Experimental eye research · 2018Article
- The evolution and expression of the snaR family of small non-coding RNAs.Nucleic acids research · 2011Article
- PHF10 is required for cell proliferation in normal and SV40-immortalized human fibroblast cells.Cytogenetic and genome research · 2009Article
- Functional identification of a BAC clone from 16q24 carrying a senescence gene SEN16 for breast cancer cells.Oncogene · 2005Article
- Article
- Identification of a gene at 16q24.3 that restores cellular senescence in immortal mammary tumor cells.Oncogene · 1999Article
- Mechanism of immortalization.Age · 1999Article
- Physiological and cytogenetic characterization of immortalized human endometriotic cells containing episomal simian virus 40 DNA.The American journal of pathology · 1999Article
- Apoptosis in Helicobacter pylori-associated gastric and duodenal ulcer disease is mediated via the Fas antigen pathway.Digestive diseases and sciences · 1999Article
- Allelotype analysis of adenocarcinoma of the gastric cardia.British journal of cancer · 1997Article
- The genetic analysis of ovarian cancer.British journal of cancer · 1995Review
- A radiation hybrid panel for human chromosome 6q.Mammalian genome : official journal of the International Mammalian Genome Society · 1995Article
- Senescence of immortal human fibroblasts by the introduction of normal human chromosome 6.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Human diploid fibroblasts have a limited life span in vitro, and spontaneous immortalization is an extremely rare event. We have used transformation of human diploid fibroblasts by an origin-defective simian virus 40 genome to develop series of genetically matched immortal cell lines to analyze immortalization. Comparison of a preimmortal transformant (SVtsA/HF-A) with its uncloned and cloned immortalized derivatives (AR5 and HAL) has failed to reveal any major alteration involving the simian virus 40 genome. Karyotypic analysis, however, demonstrated that all of the immortal cell lines in this series have alterations of chromosome 6 involving loss of the portion distal to 6q21. The karyotypic analysis was corroborated by DNA analyses. Southern analysis demonstrated that only one copy of three proto-oncogene loci (ros1, c-myb, and mas1) on 6q was retained in immortal cells. Polymerase chain reaction analysis of the microsatellite polymorphism at 6q22 (D6S87) showed loss of heterozygosity. In addition, elevated expression of c-myb (6q22-23) was observed. We hypothesize that the region at and/or distal to 6q21 plays a role in immortalization, consistent with the presence of a growth suppressor gene.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.