ArticleMolecular biology of the cell1992
Purification of a multiprotein complex containing centrosomal proteins from the Drosophila embryo by chromatography with low-affinity polyclonal antibodies.
Article in Molecular biology of the cell, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 92 citations in OpenAlex.
- NuSAP Safeguards Centriole Integrity to Mediate CEP57-CEP152 Torus Recruitment for Proper Engagement.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The MADF-BESS Protein CP60 Is Recruited to Insulators via CP190 and Has Redundant Functions inInternational journal of molecular sciences · 2023Article
- Essential role of Cp190 in physical and regulatory boundary formation.Science advances · 2022Article
- The Centrioles, Centrosomes, Basal Bodies, and Cilia ofGenetics · 2017Review
- Newly Characterized Region of CP190 Associates with Microtubules and Mediates Proper Spindle Morphology in Drosophila Stem Cells.PloS one · 2015Article
- Specific Cooperation Between Imp-α2 and Imp-β/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions.G3 (Bethesda, Md.) · 2012Article
- Dilatory is a Drosophila protein related to AZI1 (CEP131) that is located at the ciliary base and required for cilium formation.Journal of cell science · 2011Article
- The Zds proteins control entry into mitosis and target protein phosphatase 2A to the Cdc25 phosphatase.Molecular biology of the cell · 2011Article
- A microtubule interactome: complexes with roles in cell cycle and mitosis.PLoS biology · 2008Article
- Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1.The Journal of cell biology · 2008Article
- Distinct Dgrip84 isoforms correlate with distinct gamma-tubulins in Drosophila.Molecular biology of the cell · 2008Article
- Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions.Molecular biology of the cell · 2003Article
- Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae.Molecular biology of the cell · 2003Article
- Cell cycle-dependent assembly of a Gin4-septin complex.Molecular biology of the cell · 2002Article
- The Sda1 protein is required for passage through start.Molecular biology of the cell · 2001Article
- Orbit, a novel microtubule-associated protein essential for mitosis in Drosophila melanogaster.The Journal of cell biology · 2000Article
- D-TACC: a novel centrosomal protein required for normal spindle function in the early Drosophila embryo.The EMBO journal · 2000Article
- The elm1 kinase functions in a mitotic signaling network in budding yeast.Molecular and cellular biology · 1999Article
- mini spindles: A gene encoding a conserved microtubule-associated protein required for the integrity of the mitotic spindle in Drosophila.The Journal of cell biology · 1999Article
- Characterization of two related Drosophila gamma-tubulin complexes that differ in their ability to nucleate microtubules.The Journal of cell biology · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A 190-kDa centrosomal protein interacts with microtubules when Drosophila embryo extracts are passed over microtubule-affinity columns. We have obtained a partial cDNA clone that encodes this protein. Using a fusion protein produced from the clone, we have developed a novel immunoaffinity chromatography procedure that allows both the 190-kDa protein and a complex of proteins that associates with it to be isolated in in a single step. For this procedure, the fusion protein is used as an antigen to prepare rabbit polyclonal antibodies, and those antibodies that recognize the 190-kDa protein with low affinity are selectively purified on a column containing immobilized antigen. These low-affinity antibodies are then used to construct an immunoaffinity column. When Drosophila embryo extracts are passed over this column, the 190-kDa protein is quantitatively retained and can be eluted in nearly pure form under nondenaturing conditions with 1.5 M MgCl2, pH 7.6. The immunoaffinity column is washed with 1.0 M KCl just before the elution with 1.5 M MgCl2. This wash elutes 10 major proteins, as well as a number of minor ones. We present evidence that these KCl-eluted proteins represent additional centrosomal components that interact with the 190-kDa protein to form a multiprotein complex within the cell.
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