ArticleInfection and immunity1992
Cloning and expression of the gene for the Avi-3 antigen of Mycobacterium avium and mapping of its epitopes.
Article in Infection and immunity, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 22 citations in OpenAlex.
- Structural and biochemical characterization of peroxiredoxin Qbeta from Xylella fastidiosa: catalytic mechanism and high reactivity.The Journal of biological chemistry · 2010Article
- Silencing of oxidative stress response in Mycobacterium tuberculosis: expression patterns of ahpC in virulent and avirulent strains and effect of ahpC inactivation.Infection and immunity · 2001Article
- Alkyl hydroperoxide reductases C and D are major antigens constitutively expressed by Mycobacterium avium subsp. paratuberculosis.Infection and immunity · 2000Article
- Oxidative stress response and characterization of the oxyR-ahpC and furA-katG loci in Mycobacterium marinum.Journal of bacteriology · 1998Article
- Identification of epitopes of fibronectin attachment protein (FAP-A) of Mycobacterium avium which stimulate strong T-cell responses in mice.Infection and immunity · 1998Article
- Interactions of OxyR with the promoter region of the oxyR and ahpC genes from Mycobacterium leprae and Mycobacterium tuberculosis.Journal of bacteriology · 1997Article
- Processing and presentation of an antigen of Mycobacterium avium require access to an acidified compartment with active proteases.Infection and immunity · 1996Article
- Preparation of mycobacterial DNA from blood culture fluids by simple alkali wash and heat lysis method for PCR detection.Journal of clinical microbiology · 1996Article
- Oxidative stress response and its role in sensitivity to isoniazid in mycobacteria: characterization and inducibility of ahpC by peroxides in Mycobacterium smegmatis and lack of expression in M. aurum and M. tuberculosis.Journal of bacteriology · 1996Article
- Mapping of the delayed-type hypersensitivity-inducing epitope of secreted protein MPT64 from Mycobacterium tuberculosis.Infection and immunity · 1995Article
- Disparate responses to oxidative stress in saprophytic and pathogenic mycobacteria.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Cloning and sequencing of thiol-specific antioxidant from mammalian brain: alkyl hydroperoxide reductase and thiol-specific antioxidant define a large family of antioxidant enzymes.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Search for the molecular basis of morphological variation in Mycobacterium avium.Infection and immunity · 1994Article
- Antibodies to Mycobacterium paratuberculosis-specific protein antigens in Crohn's disease.Clinical and experimental immunology · 1992Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Avi-3 antigen, which is found only in Mycobacterium avium culture sonic extracts, is species specific and results in strong skin test activity in guinea pigs sensitized with heat-killed M. avium. Its gene was cloned by using a previously developed single-probe method and was sequenced. The gene encoded a 194-amino-acid polypeptide with a molecular weight of 21,500. A recombinant Avi-3 antigen expressed in Escherichia coli reacted with monoclonal and polyclonal antibodies raised against the native Avi-3 antigen. To identify epitopes on this protein for immunodiagnostic purposes, various parts of the Avi-3 antigen were expressed as beta-galactosidase fusion proteins, using pUR and pURS expression vectors. The clones screened by both antibody reactivity and T-cell proliferative activity defined fragments with coexisting B- and T-cell epitopes. A B-cell epitope (Asn-176 to Ala-186) and two T-cell epitopes (Glu-75 to Ile-86 and Arg-155 to Leu-164) were thus defined. The synthetic polymerized peptides of the T-cell epitopes were proven to elicit a delayed cutaneous hypersensitivity reaction in guinea pigs. This mapping method would be useful in the development of a subunit vaccine consisting of an immunodominant B-cell epitope linked to a T-cell epitope in the vicinity.
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