ArticleJournal of virology1992
High-frequency intracellular transposition of a defective mammalian provirus detected by an in situ colorimetric assay.
Article in Journal of virology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Tracing Mobile DNAs: From Molecular to Population Scales.Frontiers in plant science · 2022Review
- Infection barriers to successful xenotransplantation focusing on porcine endogenous retroviruses.Clinical microbiology reviews · 2012Review
- The L1 retrotransposition assay: a retrospective and toolkit.Methods (San Diego, Calif.) · 2009Review
- Identification of endogenous retroviral reading frames in the human genome.Retrovirology · 2004Article
- A Tetrahymena thermophila ribozyme-based indicator gene to detect transposition of marked retroelements in mammalian cells.Nucleic acids research · 2002Article
- Tumor cells expressing a retroviral envelope escape immune rejection in vivo.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Functional differences between the human LINE retrotransposon and retroviral reverse transcriptases for in vivo mRNA reverse transcription.The EMBO journal · 1997Article
- Expression of human immunodeficiency virus type 1 reverse transcriptase in trans during virion release and after infection.Journal of virology · 1996Article
- The dimerization/packaging sequence is dispensable for both the formation of high-molecular-weight RNA complexes within retroviral particles and the synthesis of proviruses of normal structure.Journal of virology · 1995Article
- Retrotransposition and herpesvirus evolution.Virus genes · 1995Review
- Retrotransposition of a marked Drosophila line-like I element in cells in culture.Nucleic acids research · 1994Article
- Review
- Generation of processed pseudogenes in murine cells.The EMBO journal · 1993Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We devised an indicator gene for retrotransposition, nlsLacZRT, which contains the Escherichia coli lacZ gene fused to a nuclear location signal (nlsLacZ), engineered in such a way that the gene is expressed only if the structure in which it has been inserted transposes itself through an RNA intermediate. A cloned murine leukemia retrovirus with an ecotropic host range (Moloney murine leukemia virus), rendered defective by a large deletion encompassing the three viral gag, pol, and env open reading frames, was marked with this indicator gene and introduced by transfection into heterologous feline cells. No beta-galactosidase activity could be detected among the clonal cell population, unless the defective provirus was complemented in trans by the gag-pol gene products. Under these conditions, cell variants which disclosed an easily detectable nuclear blue coloration upon in situ 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside staining were observed. Fluorescence-activated cell sorting of the beta-galactosidase-positive cells, followed by Southern blot analysis, demonstrated an unambiguous correlation between nlsLacZRT activation and retrotransposition of the marked provirus. Transposition occurs at a high frequency (up to 10(-4) events per cell per generation), which is dependent on the level of expression of the gag-pol gene and is concomitant with the release of noninfectious retroviruslike particles which are the hallmarks, but not the intermediates, of the intracellular transposition process.
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