ArticleThe Journal of cell biology1992
Binding of monoclonal antibody AA4 to gangliosides on rat basophilic leukemia cells produces changes similar to those seen with Fc epsilon receptor activation.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- Sphingolipids and their enigmatic role in asthma.Advances in biological regulation · 2018Review
- Article
- Cross-Linking Mast Cell Specific Gangliosides Stimulates the Release of Newly Formed Lipid Mediators and Newly Synthesized Cytokines.Mediators of inflammation · 2016Article
- rPbPga1 from Paracoccidioides brasiliensis Activates Mast Cells and Macrophages via NFkB.PLoS neglected tropical diseases · 2015Article
- Activation of Cdc42 is necessary for sustained oscillations of Ca2+ and PIP2 stimulated by antigen in RBL mast cells.Biology open · 2014Article
- Rat embryonic mast cells originate in the AGM.PloS one · 2013Article
- In vitro modelling of rat mucosal mast cell function in Trichinella spiralis infection.Parasite immunology · 2013Article
- GD1b-derived gangliosides modulate FcεRI endocytosis in mast cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2011Article
- Regulation and function of syk tyrosine kinase in mast cell signaling and beyond.Journal of signal transduction · 2011Article
- Lipid rafts in mast cell biology.Journal of lipids · 2011Article
- Mast cell repopulation of the peritoneal cavity: contribution of mast cell progenitors versus bone marrow derived committed mast cell precursors.BMC immunology · 2010Article
- The lectin ArtinM induces recruitment of rat mast cells from the bone marrow to the peritoneal cavity.PloS one · 2010Article
- Gangliosides are important for the preservation of the structure and organization of RBL-2H3 mast cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Article
- Mutant RBL mast cells defective in Fc epsilon RI signaling and lipid raft biosynthesis are reconstituted by activated Rho-family GTPases.Molecular biology of the cell · 2000Article
- The role of globo-series glycolipids in neuronal cell differentiation--a review.Neurochemical research · 1998Review
- Compartmentalized IgE receptor-mediated signal transduction in living cells.The Journal of cell biology · 1997Article
- The secretion-coupled endocytosis correlates with membrane tension changes in RBL 2H3 cells.The Journal of general physiology · 1997Article
- Fc epsilon RI-mediated association of 6-micron beads with RBL-2H3 mast cells results in exclusion of signaling proteins from the forming phagosome and abrogation of normal downstream signaling.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mAb AA4 binds to novel derivatives of the ganglioside Gd1b on rat basophilic leukemia (RBL-2H3) cells. Some of the gangliosides are located close to the high affinity IgE receptor (Fc epsilon RI), and binding of mAb AA4 inhibits Fc epsilon RI-mediated histamine release. In the present study, mAb AA4 was found to bind exclusively to mast cells in all rat tissues examined. In vitro, within 1 min of mAb AA4 binding, the cells underwent striking morphologic changes. They lost their normal spindle shaped appearance, increased their ruffling, and spread over the surface of the culture dish. These changes were accompanied by a redistribution of the cytoskeletal elements, actin, tubulin, and vimentin, but only the actin was associated with the membrane ruffles. Binding of mAb AA4 also induces a rise in intracellular calcium, stimulates phosphatidyl inositol breakdown, and activates PKC. However, the extent of these changes was less than that observed when the cells were stimulated with antigen or antibody directed against the Fc epsilon RI. None of these changes associated with mAb AA4 binding were seen when the cells were exposed to nonspecific IgG, IgE, or four other anti-cell surface antibodies, nor were the changes induced by binding mAb AA4 at 4 degrees C or in the absence of extracellular calcium. Although mAb AA4 does not stimulate histamine release, it enhances the effect of the calcium ionophore A23187 mediated release. The morphological and biochemical effects produced by mAb AA4 are similar to those seen following activation of the cell through the IgE receptor. Therefore, the surface gangliosides which bind mAb AA4 may function in modulating secretory events.
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