ArticleThe Journal of cell biology1992
Nuclear protein import is inhibited by an antibody to a lumenal epitope of a nuclear pore complex glycoprotein.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 98 citations in OpenAlex.
- Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations.Frontiers in immunology · 2018Article
- Detainment of Tam3 Transposase at Plasma Membrane by Its BED-Zinc Finger Domain.Plant physiology · 2017Article
- A change in nuclear pore complex composition regulates cell differentiation.Developmental cell · 2012Article
- Calcium regulation of nucleocytoplasmic transport.Protein & cell · 2011Review
- Flexible gates: dynamic topologies and functions for FG nucleoporins in nucleocytoplasmic transport.Eukaryotic cell · 2009Review
- Centrin 2 localizes to the vertebrate nuclear pore and plays a role in mRNA and protein export.Molecular and cellular biology · 2008Article
- Hormonal regulation of nuclear permeability.The Journal of biological chemistry · 2007Article
- Nuclear pore complex function in Saccharomyces cerevisiae is influenced by glycosylation of the transmembrane nucleoporin Pom152p.Genetics · 2005Article
- Transient permeability leak of nuclear envelope induced by aldosterone.The Journal of membrane biology · 2004Article
- Regulation of nuclear pore complex conformation by IP(3) receptor activation.Biophysical journal · 2002Article
- Interference with the cytoplasmic tail of gp210 disrupts "close apposition" of nuclear membranes and blocks nuclear pore dilation.The Journal of cell biology · 2002Article
- An essential nuclear envelope integral membrane protein, Brr6p, required for nuclear transport.The EMBO journal · 2001Article
- Calcium, ATP and nuclear pore channel gating.Pflugers Archiv : European journal of physiology · 2000Article
- Conformational changes of the in situ nuclear pore complex.Biophysical journal · 1999Article
- Active nuclear import and export is independent of lumenal Ca2+ stores in intact mammalian cells.The Journal of general physiology · 1999Article
- The nuclear pore complex.Plant molecular biology · 1998Review
- Involvement of p62 nucleoporin in angiotensin II-induced nuclear translocation of STAT3 in brain neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Calmodulin activates nuclear protein import: a link between signal transduction and nuclear transport.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- A yeast acetyl coenzyme A carboxylase mutant links very-long-chain fatty acid synthesis to the structure and function of the nuclear membrane-pore complex.Molecular and cellular biology · 1996Article
- Depletion of calcium from the lumen of endoplasmic reticulum reversibly inhibits passive diffusion and signal-mediated transport into the nucleus.The Journal of cell biology · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gp210 is a major transmembrane glycoprotein associated with the nuclear pore complex that is suggested to be important for organizing pore complex architecture and assembly. A mouse monoclonal IgG directed against an epitope in the lumenal domain of rat gp210 was expressed in cultured rat cells by microinjection of mRNA prepared from a hybridoma cell line. The expressed IgG, which becomes assembled into a functional antibody in the lumen of the endoplasmic reticulum, bound to the nuclear envelope in vivo. Expression of anti-gp210 antibody in interphase cells specifically reduced approximately fourfold the mediated nuclear import of a microinjected nuclear protein (nucleoplasmin) coupled to gold particles. The antibody also significantly decreased nuclear influx of a 10-kD dextran by passive diffusion. This transport inhibition did not result from removal of pore complexes from nuclear membranes or from gross alterations in pore complex structure, as shown by EM and immunocytochemistry. A physiological consequence of this transport inhibition was inhibition of cell progression from G2 into M phase. Hence, binding of this antibody to the lumenal side of gp210 must have a transmembrane effect on the structure and functions of the pore complex. These data argue that gp210 is directly or indirectly connected to pore complex constituents involved in mediated import and passive diffusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.