ArticleMolecular genetics and genomics : MGG2003
Autocrine activation of the pheromone response pathway in matalpha2- cells is attenuated by SST2- and ASG7-dependent mechanisms.
Article in Molecular genetics and genomics : MGG, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- Article
- Haploinsufficiency of the sex-determining genes atiScience · 2022Article
- Mechanisms that ensure monogamous mating inMolecular biology of the cell · 2021Article
- Heterotrimeric G Protein-coupled Receptor Signaling in Yeast Mating Pheromone Response.The Journal of biological chemistry · 2016Review
- Asymmetry in sexual pheromones is not required for ascomycete mating.Current biology : CB · 2011Article
- Multiple signals converge on a differentiation MAPK pathway.PLoS genetics · 2010Article
- How a mycoparasite employs g-protein signaling: using the example of trichoderma.Journal of signal transduction · 2010Article
- Magnificent seven: roles of G protein-coupled receptors in extracellular sensing in fungi.FEMS microbiology reviews · 2008Review
- Interspecies variation reveals a conserved repressor of alpha-specific genes in Saccharomyces yeasts.Genes & development · 2008Article
- Review
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2 authors at 1 institution in 1 country.
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Abstract
Yeast mat alpha2 mutants express both mating pheromones and both mating pheromone receptors. They show modest signaling in the pheromone response pathway, as revealed by increased levels of FUS1 transcript, yet are resistant to pheromone treatment. Together, these phenotypes suggest that alpha2- cells undergo autocrine activation of the pheromone response pathway, which is subsequently attenuated. We constructed a regulatable version of the alpha2 gene (GALalpha2) and showed that, upon loss of alpha2 activity, cells exhibit an initial robust response to pheromone that is attenuated within 3 h. We reasoned that the viability of alpha2- cells might be due to attenuation, and therefore performed a genome-wide synthetic lethal screen to identify potential adaptation components. We identified two genes, SST2 and ASG7. Loss of either of these attenuation components results in activation of the pheromone pathway in alpha2- cells. Loss of both proteins causes a more severe phenotype. Sst2 functions as a GTPase activating protein (GAP) for the Galpha subunit of the trimeric G protein. Asg7 is an a -cell specific protein that acts in concert with the alpha-cell specific a -factor receptor, Ste3, to inhibit signaling by Gbetagamma. Hence, our results suggest that mat alpha2 mutants mimic the intracellular signaling events that occur in newly fused zygotes.
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