ArticleMedical microbiology and immunology2004
An ospA-polymerase chain reaction/restriction fragment length polymorphism-based method for sensitive detection and reliable differentiation of all European Borrelia burgdorferi sensu lato species and OspA types.
Article in Medical microbiology and immunology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 69 citations in OpenAlex.
- Development of a sequence-basedMicrobial genomics · 2024Article
- Borrelia bavariensis in Questing Ixodes ricinus Ticks, United Kingdom.Emerging infectious diseases · 2023Article
- Epidemiological Surveillance of Lyme Borreliosis in Bavaria, Germany, 2013-2020.Microorganisms · 2021Article
- Recent strategies for the diagnosis of early Lyme disease.Science progress · 2018Review
- Ticks and the city - are there any differences between city parks and natural forests in terms of tick abundance and prevalence of spirochaetes?Parasites & vectors · 2017Article
- Characterization of the early local immune response to Ixodes ricinus tick bites in human skin.Experimental dermatology · 2017Article
- Immunohistochemistry and real-time PCR as diagnostic tools for detection of Borrelia burgdorferi sensu lato in ticks collected from humans.Experimental & applied acarology · 2016Article
- Oligoarthritis caused by Borrelia bavariensis, Austria, 2014.Emerging infectious diseases · 2015Review
- Comparison of detection of Borrelia burgdorferi DNA and anti-Borrelia burgdorferi antibodies in patients with erythema migrans in north-eastern Poland.Postepy dermatologii i alergologii · 2015Article
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- flaB gene as a molecular marker for distinct identification of Borrelia species in environmental samples by the PCR-restriction fragment length polymorphism method.Applied and environmental microbiology · 2011Article
- Population genetics, taxonomy, phylogeny and evolution of Borrelia burgdorferi sensu lato.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2011Review
- Reservoir competence of various rodents for the lyme disease Spirochete Borrelia spielmanii.Applied and environmental microbiology · 2011Article
- Prevalence of Borrelia burgdorferi infection in a series of 98 primary cutaneous lymphomas.The oncologist · 2011Article
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- Prevalence and seasonality of tick-borne pathogens in questing Ixodes ricinus ticks from Luxembourg.Applied and environmental microbiology · 2010Article
- Public health threat of new, reemerging, and neglected zoonoses in the industrialized world.Emerging infectious diseases · 2010Review
- A new Borrelia species defined by multilocus sequence analysis of housekeeping genes.Applied and environmental microbiology · 2009Article
- Variations in Ixodes ricinus density and Borrelia infections associated with cattle introduced into a woodland in The Netherlands.Applied and environmental microbiology · 2008Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a sensitive and reliable method for detection and differentiation of the five relevant European Borrelia burgdorferi sensu lato species ( B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. valaisiana, and B. lusitaniae), based on a heminested ospA-PCR followed by restriction enzyme analysis. Sensitivity was one borrelia per PCR except for B. afzelii, where it was five per PCR. None of seven relapsing fever borreliae, eight Leptospira serovars or two Treponema species were amplified. Except B. garinii, each of the five B. burgdorferi s.l. species is represented by one or two characteristic restriction fragment length polymorphism (RFLP) patterns. Analysis of the heterogeneous group of B. garinii resulted in five different RFLP patterns, corresponding to the OspA types 3-7 associated with this species. In a pilot study on 529 Ixodes ricinus ticks from three different regions in Southern Germany, all species and OspA types were found except B. lusitaniae and B. garinii OspA type 7, arguing for a broad distribution of almost all OspA types. A further notable finding was the focal prevalence of OspA type 4, which has rarely been detected in ticks previously. Thus, the developed method provides a fast and simple tool for epidemiological studies on the heterogeneity of species and OspA types in Europe which has important implications for the development of vaccines and (microbiological) test systems for Europe.
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