ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2004
Medicago truncatula EST-SSRs reveal cross-species genetic markers for Medicago spp.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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99 citing papers in PubMed.
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- LegumeSSRdb: A Comprehensive Microsatellite Marker Database of Legumes for Germplasm Characterization and Crop Improvement.International journal of molecular sciences · 2021Article
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- Characterization and Development of Microsatellite Markers inFrontiers in genetics · 2020Article
- Development of EST-SSR markers and their application in an analysis of the genetic diversity of the endangered species Magnolia sinostellata.Molecular genetics and genomics : MGG · 2019Article
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- Characterization of leaf transcriptome, development and utilization of unigenes-derived microsatellite markers in sugarcane (Physiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Dissecting Key Adaptation Traits in the Polyploid PerennialFrontiers in plant science · 2018Article
- Cross-species transferability of EST-SSR markers developed from the transcriptome of Melilotus and their application to population genetics research.Scientific reports · 2017Article
- Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping.BMC plant biology · 2017Article
- De novo sequencing and comparative analysis of leaf transcriptomes of diverse condensed tannin-containing lines of underutilized Psophocarpus tetragonolobus (L.) DC.Scientific reports · 2017Article
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Abstract
Expressed sequence tags (ESTs) are important resources for gene discovery and molecular marker development. From over 147,000 ESTs of Medicago truncatula, we have identified 4,384 ESTs containing perfect simple sequence repeats (EST-SSR) of di-, tri-, tetra- or pentanucleotides. Six hundred sixteen primer pairs (PPs) were designed and screened over a panel of eight genotypes representing six Medicago spp. and subspecies. Nearly, 74% (455) of the PPs produced characteristic SSR bands of expected size length in at least one Medicago species. Four hundred six (89%) of these 455 PPs produced SSR bands in all eight genotypes tested. Only 17 PPs were M. truncatula -specific. High levels of polymorphism (>70%) were detected for these markers in alfalfa, M. truncatula, and other annual medics. About 48% of the reported markers are part of gene transcripts linked to putative functions. Our results indicate that the SSR markers developed from M. truncatula ESTs are valuable genetic markers for the Medicago genus. These markers will be useful in establishing the genomic relationships of M. truncatula to important forage legume crops such as alfalfa and other annual medics.
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