ArticleMolecular biology of the cell2003
Orientational order of the lamellipodial actin network as demonstrated in living motile cells.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Integrins and tetraspanins mediate CD36-actin coupling and regulate CD36 organization and signaling.The Journal of cell biology · 2026Article
- 4polar3D single molecule imaging of 3D orientation in dense actin networks using ratiometric polarization splitting.Nature communications · 2026Article
- Quantifying cytoskeletal organization from optical microscopy data.Frontiers in cell and developmental biology · 2023Review
- A network of mixed actin polarity in the leading edge of spreading cells.Communications biology · 2022Article
- Label-free monitoring of spatiotemporal changes in the stem cell cytoskeletons in time-lapse phase-contrast microscopy.Biomedical optics express · 2022Article
- Leading edge maintenance in migrating cells is an emergent property of branched actin network growth.eLife · 2022Article
- 4polar-STORM polarized super-resolution imaging of actin filament organization in cells.Nature communications · 2022Article
- Self-organization in amoeboid motility.Frontiers in cell and developmental biology · 2022Review
- Enhanced Dynamics of Confined Cytoskeletal Filaments Driven by Asymmetric Motors.Biophysical journal · 2017Article
- Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins.Cellular microbiology · 2017Article
- Morphological Transformation and Force Generation of Active Cytoskeletal Networks.PLoS computational biology · 2017Article
- Myosin lever arm directs collective motion on cellular actin network.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Actin mediates the nanoscale membrane organization of the clustered membrane protein influenza hemagglutinin.Biophysical journal · 2013Article
- Cell migration.Comprehensive Physiology · 2012Review
- Actin filament curvature biases branching direction.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Actin bends over backward for directional branching.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Simulation of lamellipodial fragments.Journal of mathematical biology · 2012Article
- Actin filament elasticity and retrograde flow shape the force-velocity relation of motile cells.Biophysical journal · 2012Article
- Inferring biological structures from super-resolution single molecule images using generative models.PloS one · 2012Article
- Integrin adhesion and force coupling are independently regulated by localized PtdIns(4,5)2 synthesis.The EMBO journal · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Lamellipodia of crawling cells represent both the motor for cell advance and the primary building site for the actin cytoskeleton. The organization of actin in the lamellipodium reflects actin dynamics and is of critical importance for the mechanism of cell motility. In previous structural studies, the lamellipodial actin network was analyzed primarily by electron microscopy (EM). An understanding of lamellipodial organization would benefit significantly if the EM data were complemented and put into a kinetic context by establishing correspondence with structural features observable at the light microscopic level in living cells. Here, we use an enhanced phase contrast microscopy technique to visualize an apparent long-range diagonal actin meshwork in the advancing lamellipodia of living cells. Visualization of this meshwork permitted a correlative light and electron microscopic approach that validated the underlying organization of lamellipodia. The linear features in the light microscopic meshwork corresponded to regions of greater actin filament density. Orientation of features was analyzed quantitatively and compared with the orientation of actin filaments at the EM level. We infer that the light microscopic meshwork reflects the orientational order of actin filaments which, in turn, is related to their branching angle.
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