ArticleTissue engineering2003
Platelet-derived growth factor gene delivery stimulates ex vivo gingival repair.
Article in Tissue engineering, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 63 citations in OpenAlex.
- Multifunctional DNA-Collagen Biomaterials: Developmental Advances and Biomedical Applications.ACS biomaterials science & engineering · 2025Review
- Effect of PDGF-B Gene-Activated Acellular Matrix and Mesenchymal Stem Cell Transplantation on Full Thickness Skin Burn Wound in Rat Model.Tissue engineering and regenerative medicine · 2021Article
- Gene Delivery Therapeutics in the Treatment of Periodontitis and Peri-Implantitis: A State of the Art Review.International journal of molecular sciences · 2019Review
- Biomaterials, Current Strategies, and Novel Nano-Technological Approaches for Periodontal Regeneration.Journal of functional biomaterials · 2019Review
- Effect of sustained PDGF nonviral gene delivery on repair of tooth-supporting bone defects.Gene therapy · 2017Article
- Integration of growth factor gene delivery with collagen-triggered wound repair cascades using collagen-mimetic peptides.Bioengineering & translational medicine · 2016Article
- A Pilot Study Evaluating Combinatorial and Simultaneous Delivery of Polyethylenimine-Plasmid DNA Complexes Encoding for VEGF and PDGF for Bone Regeneration in Calvarial Bone Defects.Current pharmaceutical biotechnology · 2015Article
- Article
- Gene therapy in periodontics.Journal of Indian Society of Periodontology · 2013Article
- Role of platelet-rich-fibrin in enhancing palatal wound healing after free graft.Contemporary clinical dentistry · 2012Article
- Advanced reconstructive technologies for periodontal tissue repair.Periodontology 2000 · 2012Review
- Lysophosphatidic Acid signals through specific lysophosphatidic Acid receptor subtypes to control key regenerative responses of human gingival and periodontal ligament fibroblasts.Journal of periodontology · 2009Article
- Gene therapy and its implications in Periodontics.Journal of Indian Society of Periodontology · 2009Article
- Platelet-derived growth factor-B gene delivery sustains gingival fibroblast signal transduction.Journal of periodontal research · 2008Article
- Craniofacial tissue engineering by stem cells.Journal of dental research · 2006Review
- Growth factor delivery for oral and periodontal tissue engineering.Expert opinion on drug delivery · 2006Review
- Nano-fibrous scaffold for controlled delivery of recombinant human PDGF-BB.Journal of controlled release : official journal of the Controlled Release Society · 2006Article
- Gene therapeutics for periodontal regenerative medicine.Dental clinics of North America · 2006Review
- Current concepts in periodontal bioengineering.Orthodontics & craniofacial research · 2005Review
- Engineering of tooth-supporting structures by delivery of PDGF gene therapy vectors.Molecular therapy : the journal of the American Society of Gene Therapy · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Destruction of tooth support due to the chronic inflammatory disease periodontitis is a major cause of tooth loss. There are limitations with available treatment options to tissue engineer soft tissue periodontal defects. The exogenous application of growth factors (GFs) such as platelet-derived growth factor (PDGF) has shown promise to enhance oral and periodontal tissue regeneration. However, the topical administration of GFs has not led to clinically significant improvements in tissue regeneration because of problems in maintaining therapeutic protein levels at the defect site. The utilization of PDGF gene transfer may circumvent many of the limitations with protein delivery to soft tissue wounds. The objective of this study was to test the effect of PDGF-A and PDGF-B gene transfer to human gingival fibroblasts (HGFs) on ex vivo repair in three-dimensional collagen lattices. HGFs were transduced with adenovirus encoding PDGF-A and PDGF-B genes. Defect fill of bilayer collagen gels was measured by image analysis of cell repopulation into the gingival defects. The modulation of gene expression at the defect site and periphery was measured by RT-PCR during a 10-day time course after gene delivery. The results demonstrated that PDGF-B gene transfer stimulated potent (>4-fold) increases in cell repopulation and defect fill above that of PDGF-A and corresponding controls. PDGF-A and PDGF-B gene expression was maintained for at least 10 days. PDGF gene transfer upregulated the expression of phosphatidylinosital 3-kinase and integrin alpha5 subunit at 5 days after adenovirus transduction. These results suggest that PDGF gene transfer has potential for periodontal soft tissue-engineering applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.