ArticlePlant molecular biology2003
Disruption of plE2, the gene for the E2 subunit of the plastid pyruvate dehydrogenase complex, in Arabidopsis causes an early embryo lethal phenotype.
Article in Plant molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 64 citations in OpenAlex.
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- Proximity labelling suggests association of the nonhost receptor PSS1 protein with enzymes of multiple defense pathways in Arabidopsis.Frontiers in plant science · 2025Article
- Coordination of mPlants (Basel, Switzerland) · 2023Article
- GWAS on multiple traits identifies mitochondrial ACONITASE3 as important for acclimation to submergence stress.Plant physiology · 2022Article
- Transcriptome analysis reveals key developmental and metabolic regulatory aspects of oil palm (Elaeis guineensis Jacq.) during zygotic embryo development.BMC plant biology · 2022Article
- Differential Activation of Partially Redundant Δ9 Stearoyl-ACP Desaturase Genes Is Critical for Omega-9 Monounsaturated Fatty Acid Biosynthesis During Seed Development in Arabidopsis.The Plant cell · 2020Article
- Failure to Maintain Acetate Homeostasis by Acetate-Activating Enzymes Impacts Plant Development.Plant physiology · 2020Article
- Impact of sunflower (Helianthus annuus L.) plastidial lipoyl synthases genes expression in glycerolipids composition of transgenic Arabidopsis plants.Scientific reports · 2020Article
- Lipidomic Analysis of Plastidial Octanoyltransferase Mutants ofMetabolites · 2019Article
- Xylan in the Middle: Understanding Xylan Biosynthesis and Its Metabolic Dependencies Toward Improving Wood Fiber for Industrial Processing.Frontiers in plant science · 2019Review
- Article
- Comparative RNA-Seq Analysis of High- and Low-Oil Yellow Horn During Embryonic Development.International journal of molecular sciences · 2018Article
- Roles and maturation of iron-sulfur proteins in plastids.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Review
- Stable, Environmental Specific and Novel QTL Identification as Well as Genetic Dissection of Fatty Acid Metabolism inFrontiers in plant science · 2018Article
- Differential gene expression at different stages of mesocarp development in high- and low-yielding oil palm.BMC genomics · 2017Article
- The mechanism of high contents of oil and oleic acid revealed by transcriptomic and lipidomic analysis during embryogenesis in Carya cathayensis Sarg.BMC genomics · 2016Article
- Comparative proteomic analyses of Asian cotton ovules with attached fibers in the early stages of fiber elongation process.Proteome science · 2016Article
- Enzymatic characterization of two acetyl-CoA synthetase genes from Populus trichocarpa.SpringerPlus · 2016Article
- Senescence-inducible LEC2 enhances triacylglycerol accumulation in leaves without negatively affecting plant growth.Plant biotechnology journal · 2015Article
- Mutations in the three Arabidopsis genes that encode the E2 subunit of the mitochondrial pyruvate dehydrogenase complex differentially affect enzymatic activity and plant growth.Plant cell reports · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pyruvate dehydrogenase multi-enzyme complex is the main source of acetyl-CoA formation in the plastids of plants and is composed of multiple copies of four different subunits, E1alpha, E1beta, E2, and E3. A T-DNA insertion into the gene for the plastidic E2 (dihydrolipoyl acetyltransferase) subunit, plE2, of the complex in Arabidopsis destroys the expression of that gene. The resulting mutation has no apparent phenotype in the heterozygous state, but the homozygous mutation is lethal. Haploid sperm and eggs that contain only the disrupted plE2 gene function normally resulting in the formation of an embryo that is homozygous for the mutation. This embryo only develops to an early stage before the development arrests resulting in an early embryo-lethal phenotype. While the mutation could not be complemented with the cDNA for the plE2 gene under control of the 35S, the AtSERK1, or the napin promoter, it could be complemented using the endogenous plE2 promoter to drive expression of the plE2 cDNA. This verifies the essential nature of the plastidic pyruvate dehydrogenase complex and its role in embryo formation.
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