ArticlePlant molecular biology2003
Transgene structures in T-DNA-inserted rice plants.
Article in Plant molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 153 citations in OpenAlex.
- T-DNA orientation, distance between two T-DNAs, and the transformation target cells significantly impact vector backbone integration and efficiency of generating marker-free transgenic plants in a co-transformation system.The Plant journal : for cell and molecular biology · 2025Article
- Genomic consequences associated with Agrobacterium-mediated transformation of plants.The Plant journal : for cell and molecular biology · 2024Review
- Characterization of integration sites and transfer DNA structures in Agrobacterium-mediated transgenic events of maize inbred B104.G3 (Bethesda, Md.) · 2023Article
- Deficiencies in the Risk Assessment of Genetically Engineered Bt Cowpea Approved for Cultivation in Nigeria: A Critical Review.Plants (Basel, Switzerland) · 2022Review
- Advances and Perspectives in Tissue Culture and Genetic Engineering of Cannabis.International journal of molecular sciences · 2021Review
- Whole-genome resequencing using next-generation and Nanopore sequencing for molecular characterization of T-DNA integration in transgenic poplar 741.BMC genomics · 2021Article
- Transformation and Characterization of Δ12-Fatty Acid Acetylenase and Δ12-Oleate Desaturase Potentially Involved in the Polyacetylene Biosynthetic Pathway fromPlants (Basel, Switzerland) · 2020Article
- Spatiotemporal Controllability and Environmental Risk Assessment of Genetically Engineered Gene Drive Organisms from the Perspective of European Union Genetically Modified Organism Regulation.Integrated environmental assessment and management · 2020Review
- Targeted Mutagenesis of the RiceInternational journal of molecular sciences · 2020Article
- Comprehensive characterization of T-DNA integration induced chromosomal rearrangement in a birch T-DNA mutant.BMC genomics · 2019Article
- Using Morphogenic Genes to Improve Recovery and Regeneration of Transgenic Plants.Plants (Basel, Switzerland) · 2019Review
- Transcriptome profiling of transgenic potato plants provides insights into variability caused by plant transformation.PloS one · 2018Article
- An Insight into T-DNA Integration Events in Medicago sativa.International journal of molecular sciences · 2017Article
- RNA-Guided Cas9-Induced Mutagenesis in Tobacco Followed by Efficient Genetic Fixation in Doubled Haploid Plants.Frontiers in plant science · 2016Article
- High-throughput transformation pipeline for a Brazilian japonica rice with bar gene selection.Protoplasma · 2015Article
- A comparative analysis of insertional effects in genetically engineered plants: considerations for pre-market assessments.Transgenic research · 2015Review
- Efficient generation of marker-free transgenic rice plants using an improved transposon-mediated transgene reintegration strategy.Plant physiology · 2015Article
- Ensifer-mediated transformation: an efficient non-Agrobacterium protocol for the genetic modification of rice.SpringerPlus · 2015Article
- A novel T-DNA integration in rice involving two interchromosomal translocations.Plant cell reports · 2014Article
- Comparison of Agrobacterium and particle bombardment using whole plasmid or minimal cassette for production of high-expressing, low-copy transgenic plants.Transgenic research · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
T-DNA is commonly used for delivery of foreign genes and as an insertional mutagen. Although ample information exists regarding T-DNA organization in dicotyledonous plants, little is known about the monocot rice. Here, we investigated the structure of T-DNA in a large number of transgenic rice plants. Analysis of the T-DNA borders revealed that more than half of the right ends were at the cleavage site, whereas the left ends were not conserved and were deleted up to 180 bp from the left border (LB) cleavage site. Three types of junctions were found between T-DNA and genomic DNA. In the first, up to seven nucleotide overlaps were present. The frequency of this type was much higher in the LB region than at the right border (RB). In the second type, which was more frequent in RB, the link was direct, without any overlaps or filler DNA. Finally, the third type showed filler DNA between T-DNA and the plant sequences. Out of 171 samples examined, 77 carried the vector backbone sequence, with the majority caused by the failure of T-strand termination at LB. However, a significant portion also resulted from co-integration of T-DNA and the vector backbone to a single locus. Most linkages between T-DNA and the vector backbone were formed between two 3' ends or two 5' ends of the transferred DNAs. The 3' ends were mostly linked through 3-6 bp of the complementing sequence, whereas the 5' ends were linked through either precise junctions or imprecise junctions with filler DNA.
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Registered trials
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