Evidence map›Paper›PMID 1358028›Full record

ArticleAntonie van Leeuwenhoek1992

Genomic studies on killer yeasts belonging to the genus Pichia.

G Morace, P Cattani, S Manzara, S Conti, M Gerloni, M Sanguinetti, B Posteraro, L Polonelli

Abstract read
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Article in Antonie van Leeuwenhoek, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

G MoraceIstituto di Microbiologia, Università Cattolica del Sacro Cuore, Roma, Italy.
P Cattani
S Manzara
S Conti
M Gerloni
M Sanguinetti
B Posteraro
L Polonelli
Università Cattolica del Sacro Cuore · ITUniversity of Parma · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Twenty-four species belonging to the genus Pichia were investigated using restriction fragment length polymorphism (RFLP) and Southern blot hybridization of their genomic DNA. Saccharomyces cerevisiae, Kluyveromyces lactis, Williopsis mrakii and Candida albicans were also included in this study. The RFLP patterns were obtained from digestion of yeast DNA with several restriction endonuclease enzymes, and showed various bands with different mobility; in most isolates, the more deeply stained bands were species-specific. This observation was confirmed by the results obtained from Southern blot hybridization of the EcoRI and XhoI RFLP patterns with P. anomala UCSC 25F DNA, digested with the same enzymes, used as probes. These bands are likely to be ribosomal DNA as shown by hybridization of digested DNA from unrelated yeast species (S. cerevisiae, K. lactis and C. albicans). However, one hybridized band, located at 3.9-4.1 Kb, seems to be peculiar to the Pichia species. Our study confirms the usefulness of molecular tools in studying genetic relatedness among yeasts.

Indexed as

Genome, FungalBlotting, SouthernDeoxyribonuclease EcoRIDeoxyribonucleases, Type II Site-SpecificDNA, FungalPichiaPolymorphism, Restriction Fragment LengthCTCGAG-specific type II deoxyribonucleasesDeoxyribonuclease EcoRIDeoxyribonucleases, Type II Site-SpecificDNA, Fungal

Identifiers

PMID1358028
OpenAlexW2067094749

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.