ArticleProceedings of the National Academy of Sciences of the United States of America1992
Somatostatin receptors are expressed by immature cerebellar granule cells: evidence for a direct inhibitory effect of somatostatin on neuroblast activity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 100 citations in OpenAlex.
- Somatostatin-Mediated Regulation of Retinoic Acid-Induced Differentiation of SH-SY5Y Cells: Neurotransmitters Phenotype Characterization.Biomedicines · 2022Article
- Paracrine Role for Somatostatin Interneurons in the Assembly of Perisomatic Inhibitory Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- GFrontiers in aging neuroscience · 2019Review
- A Peptidomic Approach to Characterize Peptides Involved in Cerebellar Cortex Development Leads to the Identification of the Neurotrophic Effects of Nociceptin.Molecular & cellular proteomics : MCP · 2018Article
- Somatostatin and Somatostatin-Containing Neurons in Shaping Neuronal Activity and Plasticity.Frontiers in neural circuits · 2016Review
- Melatonin protects rat cerebellar granule cells against electromagnetic field-induced increases in Na(+) currents through intracellular Ca(2+) release.Journal of cellular and molecular medicine · 2014Article
- Article
- Mechanisms of ischemic brain damage.Neuropharmacology · 2008Review
- Pituitary adenylate cyclase-activating polypeptide prevents the effects of ceramides on migration, neurite outgrowth, and cytoskeleton remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Pituitary adenylate cyclase-activating polypeptide protects rat cerebellar granule neurons against ethanol-induced apoptotic cell death.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The neuroprotective effect of pituitary adenylate cyclase-activating polypeptide on cerebellar granule cells is mediated through inhibition of the CED3-related cysteine protease caspase-3/CPP32.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- The epidural and intrathecal administration of somatotrophin-release inhibiting factor: native and synthetic analogues.Current review of pain · 2000Review
- Neurotrophic activity of pituitary adenylate cyclase-activating polypeptide on rat cerebellar cortex during development.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Heteromultimerization of G-protein-gated inwardly rectifying K+ channel proteins GIRK1 and GIRK2 and their altered expression in weaver brain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Occurrence of two somatostatin variants in the frog brain: characterization of the cDNAs, distribution of the mRNAs, and receptor-binding affinities of the peptides.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Somatostatin and leu-enkephalin in the rat auditory brainstem during fetal and postnatal development.Anatomy and embryology · 1995Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Somatostatin and somatostatin receptors are transiently expressed in the immature rat cerebellar cortex but virtually undetectable in the cerebellum of adults. Although somatostatin binding sites have been visualized during the postnatal period in the external granule cell layer, the type of cell that expresses somatostatin receptors has never been identified; thus, the potential function of somatostatin in the developing cerebellum remains unknown. In the present study, we have taken advantage of the possibility of obtaining a culture preparation that is greatly enriched in immature cerebellar granule cells to investigate the presence of somatostatin receptors and the effect of somatostatin on intracellular messengers on cerebellar neuroblasts in primary culture. Autoradiographic labeling revealed the occurrence of a high density of binding sites for radioiodinated Tyr-[D-Trp8]somatostatin-(1-14) on 1-day-old cultured immature granule cells. Saturation and competition studies showed the existence of a single class of high-affinity binding sites (Kd = 0.133 +/- 0.013 nM, Bmax = 3038 +/- 217 sites per cell). Somatostatin induced a dose-dependent inhibition of forskolin-evoked cAMP formation (ED50 = 10 nM), and this effect was prevented by preincubation of cultured immature granule cells with pertussis toxin. Somatostatin also caused a marked reduction of intracellular calcium concentration. These results show the presence of functionally active somatostatin receptors on immature granule cells. Our data suggest the possible involvement of somatostatin in the regulation of proliferation and/or migration of neuroblasts during the development of the cerebellar cortex.
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