ArticleJournal of bacteriology1992
Murein-metabolizing enzymes from Escherichia coli: existence of a second lytic transglycosylase.
Article in Journal of bacteriology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Lytic Transglycosylase Deficiency Increases Susceptibility to β-lactam Antibiotics But Reduces Susceptibility to Vancomycin in Escherichia coli.Microbiology and immunology · 2025Article
- Escherichia coli has robust regulatory mechanisms against elevated peptidoglycan cleavage by lytic transglycosylases.The Journal of biological chemistry · 2023Article
- A Defect in Lipoprotein Modification by Lgt Leads to Abnormal Morphology and Cell Death in Escherichia coli That Is Independent of Major Lipoprotein Lpp.Journal of bacteriology · 2022Article
- Antibacterial Activity of Sulfated Galactans fromMarine drugs · 2020Article
- A step-by-stepThe Journal of biological chemistry · 2018Article
- Attention Seeker: Production, Modification, and Release of Inflammatory Peptidoglycan Fragments in Neisseria Species.Journal of bacteriology · 2017Review
- Lytic transglycosylases: concinnity in concision of the bacterial cell wall.Critical reviews in biochemistry and molecular biology · 2017Review
- Reactions of all Escherichia coli lytic transglycosylases with bacterial cell wall.Journal of the American Chemical Society · 2013Article
- The lytic transglycosylases of Neisseria gonorrhoeae.Microbial drug resistance (Larchmont, N.Y.) · 2012Review
- Peptidoglycan hydrolases of Escherichia coli.Microbiology and molecular biology reviews : MMBR · 2011Review
- Neisseria gonorrhoeae uses two lytic transglycosylases to produce cytotoxic peptidoglycan monomers.Journal of bacteriology · 2008Article
- An anhydro-N-acetylmuramyl-L-alanine amidase with broad specificity tethered to the outer membrane of Escherichia coli.Journal of bacteriology · 2007Article
- Extragenic suppressors of growth defects in msbB Salmonella.Journal of bacteriology · 2001Article
- Linkage map of Escherichia coli K-12, edition 10: the traditional map.Microbiology and molecular biology reviews : MMBR · 1998Review
- Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli.Microbiology and molecular biology reviews : MMBR · 1998Review
- Outer membrane localization of murein hydrolases: MltA, a third lipoprotein lytic transglycosylase in Escherichia coli.Journal of bacteriology · 1997Article
- From growth to autolysis: the murein hydrolases in Escherichia coli.Archives of microbiology · 1995Review
- G(AnH)MTetra, a naturally occurring 1,6-anhydro muramyl dipeptide, induces granulocyte colony-stimulating factor expression in human monocytes: a molecular analysis.Infection and immunity · 1994Article
- Purification and properties of a membrane-bound lytic transglycosylase from Escherichia coli.Journal of bacteriology · 1994Article
- Functions of the gene products of Escherichia coli.Microbiological reviews · 1993Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In addition to the soluble lytic transglycosylase, a murein-metabolizing enzyme with a molecular mass of 70 kDa (Slt70), Escherichia coli possesses a second lytic transglycosylase, which has been described as a membrane-bound lytic transglycosylase (Mlt; 35 kDa; EC 3.2.1.-). The mlt gene, which supposedly encodes Mlt, was cloned, and the complete nucleotide sequence was determined. The open reading frame, identified on a 1.7-kb SalI-PstI fragment, codes for a protein of 323 amino acids (M(r) = 37,410). Two transmembrane helices and one membrane-associated helix were predicted in the N-terminal half of the protein. Lysine and arginine residues represent up to 15% of the amino acids, resulting in a calculated isoelectric point of 10.0. The deduced primary structure did not show significant sequence similarity to Slt70 from E. coli. High-level expression of the presumed mlt gene was not paralleled by an increase in murein hydrolase activity. To clarify the identity of the second transglycosylase, we purified an enzyme with the specificity of a transglycosylase from an E. coli slt deletion strain. The completely soluble transglycosylase, with a molecular mass of approximately 35 kDa, was designated Slt35. Its determined 26 N-terminal amino acids showed similarity to a segment in the middle of the Slt70 primary structure. Polyclonal anti-Mlt antibodies, which had been used for the isolation of the mlt gene, were found to cross-react with Mlt as well as with Slt35, suggesting that the previously described Mlt preparation was contaminated with Slt35. We conclude that the second transglycosylase of E. coli is not a membrane-bound protein but rather is a soluble protein.
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