ArticleProceedings of the National Academy of Sciences of the United States of America1992
A role for microtubules in sorting endocytic vesicles in rat hepatocytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 88 citations in OpenAlex.
- Gut Bacteria-derived Membrane Vesicles Induce Colonic Dysplasia by Inducing DNA Damage in Colon Epithelial Cells.Cellular and molecular gastroenterology and hepatology · 2024Article
- Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3): Unraveling the Role in Mediating IGF-Independent Effects Within the Cell.Frontiers in cell and developmental biology · 2020Review
- Targeting microtubules sensitizes drug resistant lung cancer cells to lysosomal pathway inhibitors.Theranostics · 2020Article
- Specific IgA Enhances the Transcytosis and Excretion of Hepatitis A Virus.Scientific reports · 2016Article
- Endocytosis‒Mediated Invasion and Pathogenicity of Streptococcus agalactiae in Rat Cardiomyocyte (H9C2).PloS one · 2015Article
- Down-regulation of desmosomes in cultured cells: the roles of PKC, microtubules and lysosomal/proteasomal degradation.PloS one · 2014Article
- Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.PloS one · 2011Article
- Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors.Science (New York, N.Y.) · 2008Article
- Single vesicle analysis of endocytic fission on microtubules in vitro.Traffic (Copenhagen, Denmark) · 2008Article
- New liver cell mutants defective in the endocytic pathway.Biochimica et biophysica acta · 2007Article
- Kif5B and Kifc1 interact and are required for motility and fission of early endocytic vesicles in mouse liver.Molecular biology of the cell · 2007Article
- Microtubule and motor-dependent endocytic vesicle sorting in vitro.The Journal of cell biology · 2000Article
- Reconstitution of ATP-dependent movement of endocytic vesicles along microtubules in vitro: an oscillatory bidirectional process.Molecular biology of the cell · 2000Article
- Increased protein phosphorylation of cytoplasmic dynein results in impaired motor function.The Biochemical journal · 1999Article
- Molecular aspects of the endocytic pathway.The Biochemical journal · 1998Review
- Annexin VI-mediated loss of spectrin during coated pit budding is coupled to delivery of LDL to lysosomes.The Journal of cell biology · 1998Article
- Gene transfection of multicellular spheroid of hepatocytes on an artificial substrate.Cytotechnology · 1998Article
- Characterization and functional studies on rat liver fat-storing cell line and freshly isolated hepatocyte coculture system.The American journal of pathology · 1995Article
- Expression of an epidermal keratin protein in liver of transgenic mice causes structural and functional abnormalities.The Journal of cell biology · 1995Article
- Molecular motors are differentially distributed on Golgi membranes from polarized epithelial cells.The Journal of cell biology · 1994Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The vectorial nature of hepatocyte receptor-mediated endocytosis (RME) and its susceptibility to cytoskeletal disruptors has suggested that a polarized network of microtubules plays a vital role in directed movement during sorting. Using as markers a well-known ligand, asialoorosomucoid, and its receptor, we have isolated endocytic vesicles that bind directly to and interact with stabilized endogenous hepatocyte microtubules at specific times during a synchronous, experimentally initiated, single wave of RME. Both ligand- and receptor-containing vesicles copelleted with microtubules in the absence of ATP but did not pellet under similar conditions when microtubules were not polymerized. When 5 mM ATP was added to preparations of microtubule-bound vesicles, ligand-containing vesicles were released into the supernatant, while receptor-containing vesicles remained immobilized on the microtubules. Release of ligand-containing vesicles from microtubules was prevented by monensin treatment during the endocytic wave. Several proteins, including the microtubule motor protein cytoplasmic dynein, were present in these preparations and were released from microtubule pellets by ATP addition concomitantly with ligand. These results suggest that receptor domains within the endosome can be immobilized by attachment to microtubules so that, following monensin-sensitive dissociation of ligand from receptor, ligand-containing vesicles can be pulled along microtubules away from the receptor domains by a motor molecule, such as cytoplasmic dynein, thereby delineating sorting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.