ArticleThe EMBO journal1992
Modulation of transcytotic and direct targeting pathways in a polarized thyroid cell line.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 101 citations in OpenAlex.
- Sonic hedgehog is basolaterally sorted from the TGN and transcytosed to the apical domain involving Dispatched-1 at Rab11-ARE.Frontiers in cell and developmental biology · 2022Article
- Prestin Contributes to Membrane Compartmentalization and Is Required for Normal Innervation of Outer Hair Cells.Frontiers in cellular neuroscience · 2018Article
- Pax8 controls thyroid follicular polarity through cadherin-16.Journal of cell science · 2017Article
- Article
- Milk secretion: The role of SNARE proteins.Journal of mammary gland biology and neoplasia · 2014Review
- Cathepsin B trafficking in thyroid carcinoma cells.Thyroid research · 2011Article
- Apical trafficking in epithelial cells: signals, clusters and motors.Journal of cell science · 2009Review
- Antiribosomal-P autoantibodies from psychiatric lupus target a novel neuronal surface protein causing calcium influx and apoptosis.The Journal of experimental medicine · 2007Article
- Cell polarity development and protein trafficking in hepatocytes lacking E-cadherin/beta-catenin-based adherens junctions.Molecular biology of the cell · 2007Article
- AP1B sorts basolateral proteins in recycling and biosynthetic routes of MDCK cells.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Controversy fuels trafficking of GPI-anchored proteins.The Journal of cell biology · 2006Article
- GPI-anchored proteins are directly targeted to the apical surface in fully polarized MDCK cells.The Journal of cell biology · 2006Article
- Functional interaction between p75NTR and TrkA: the endocytic trafficking of p75NTR is driven by TrkA and regulates TrkA-mediated signalling.The Biochemical journal · 2005Article
- Protein oligomerization modulates raft partitioning and apical sorting of GPI-anchored proteins.The Journal of cell biology · 2004Article
- Thyroid functions of mouse cathepsins B, K, and L.The Journal of clinical investigation · 2003Article
- Raft-mediated trafficking of apical resident proteins occurs in both direct and transcytotic pathways in polarized hepatic cells: role of distinct lipid microdomains.Molecular biology of the cell · 2003Article
- Characterization of a di-leucine-based signal in the cytoplasmic tail of the nucleotide-pyrophosphatase NPP1 that mediates basolateral targeting but not endocytosis.Molecular biology of the cell · 2001Article
- Polarized sphingolipid transport from the subapical compartment changes during cell polarity development.Molecular biology of the cell · 2000Article
- Detergent-insoluble GPI-anchored proteins are apically sorted in fischer rat thyroid cells, but interference with cholesterol or sphingolipids differentially affects detergent insolubility and apical sorting.Molecular biology of the cell · 2000Article
- Mechanisms and functional features of polarized membrane traffic in epithelial and hepatic cells.The Biochemical journal · 1998Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Two biosynthetic pathways exist for delivery of membrane proteins to the apical surface of epithelial cells, direct transport from the trans-Golgi network (TGN) and transcytosis from the basolateral membrane. Different epithelial cells vary in the expression of these mechanisms. Two extremes are MDCK cells, that use predominantly the direct route and hepatocytes, which deliver all apical proteins via the basolateral membrane. To determine how epithelial cells establish a particular targeting phenotype, we studied the apical delivery of endogenous dipeptidyl peptidase IV (DPPIV) at early and late stages in the development of monolayers of a highly polarized epithelial cell line derived from Fischer rat thyroid (FRT). In 1 day old monolayers, surface delivery of DPPIV from the TGN was unpolarized (50%/50%) but a large basal to apical transcytotic component resulted in a polarized apical distribution. In contrast, after 7 days of culture, delivery of DPPIV was mainly direct (85%) with no transcytosis of the missorted component. A basolateral marker, Ag 35/40 kD, on the other hand, was directly targeted (90-98%) at all times. These results indicate that the sorting machinery for apical proteins develops independently from the sorting machinery for basolateral proteins and that the sorting site relocates progressively from the basal membrane to the TGN during development of the epithelium. The transient expression of the transcytotic pathway may serve as a salvage pathway for missorted apical proteins when the polarized phenotype is being established.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.